中华羊茅Epichloё内生真菌的actin序列分析  

Analysis of actin sequences from Epichloё endophyte in Festuca sinensis

作　　者：旷宇[1] 汪建军[1] 许文博[1] 田沛[1] 

机构地区：[1]草地农业生态系统国家重点实验室,兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业学报》2016年第9期125-131,共7页Acta Prataculturae Sinica

基　　金：国家重点基础研究发展规划(973计划)(2014CB138702);国家自然科学基金(31502001);中央高校基本科研业务费(lzujbky-2016-9)资助

摘　　要：Epichloё内生真菌与禾本科植物很常见。现已有大量关于禾草Epichloё内生真菌系统演化研究的报道,但是尚未发现中华羊茅所带Epichloё内生真菌系统演化方面的研究。本研究经过检测采自青海、甘肃、四川等地中华羊茅8个种群的Epichloё内生真菌带菌率发现8个种群的Epichloё内生真菌均带有Epichloё内生真菌,带菌率为100%。该结果为研究中华羊茅所带Epichloё内生真菌系统演化的研究提供了优良的材料。因此,本研究通过克隆actin(act)序列,并与已报道Epichloё内生真菌act序列构建系统进化树。结果表明,供试菌act序列在进化树上成单支分布,表明该菌属于同一种Epichloё内生真菌,且可能是一个新种。此外,遗传距离分析也证明了供试菌为同一菌种。进化关系同时发现分布在相同或相近地理位置的Epichloё内生真菌聚集在同一个分支,表明它们之间的亲缘关系较近,表明Epichloё内生真菌亲缘关系与宿主采集地密切相关。This study detected Epichloe endophyte in the seeds of eight collections of Festuca sinensis, collected from Qinghai, Gansu, Sichuan and other parts of China, with an infection rate of 100%. This provided excel- lent material to evaluate the taxonomic status of Epichloe endophyte in F. sinensis. A phylogenetic tree was constructed with actin gene sequences by cloning from the collected F. sinensis seeds and comparison with pub- lished actin gene sequences downloaded from GenBank. The actin sequences of Epichloe endophyte strains iso- lated from the eight F. sinensis accessions were clustered together and formed a separated clade, which sug- gests that endophyte strains in different F. sinensis accessions belonged to the same taxon, but may be a new species. Moreover, population genetic distance analysis also confirmed these 8 F.pichloe strains to be conspecif- ic. The phylogentic tree also distinguished Epichloe endophyte strains isolated from F. sinensis at the same or nearby sampling sites from strains collected from more distant sites. In summary, the findings indicate a possi ble new species of Epichloe in F. sinensis, with distinct strains at geographically different sampling sites.

关 键 词：内生真菌 中华羊茅 act基因 遗传距离 系统发育 

分 类 号：S543.9[农业科学—作物学] Q93[生物学—微生物学]