日粮NDF水平对断奶獭兔血液免疫细胞与肠道特性的影响  

The effect of dietary NDF levels on blood immune system cells and intestinal characteristics of Rex rabbits at weaning

作　　者：任战军[1,2] 高玉琪[2] 王金丽[2] 李发弟[1] 

机构地区：[1]甘肃农业大学动物科技学院,甘肃兰州730070 [2]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《草业学报》2016年第9期197-203,共7页Acta Prataculturae Sinica

基　　金：陕西省农业公关项目(2010K01-16);西安市农业攻关项目(NC09049-4)资助

摘　　要：研究日粮中性洗涤纤维(NDF)水平对断奶獭兔血液免疫细胞、小肠分泌型免疫球蛋白A(sIgA)、盲肠微生物及盲肠pH的影响,为揭示日粮NDF和肠道健康关系和断奶獭兔日粮的配制提供理论依据。选择35日龄体重差异不显著的断奶獭兔56只,随机分成4组,每组14只(每只为一个重复)。分别饲喂NDF水平为29%,33%,37%,41%的日粮。预试7d,正式试验28d。试验结束后各组随机选取6只屠宰采样,测量血液及肠道各项指标。结果表明,随着日粮NDF水平提高,断奶獭兔白细胞数无显著差异(P>0.05);血液淋巴细胞数上升(P<0.05);十二指肠和空肠sIgA+细胞数显著增加(P<0.05);盲肠总细菌数无显著差异(P>0.05);拟杆菌数先增加后减少,33%组显著高于其他各组(P<0.05);乳酸菌和大肠杆菌数降低,且乳酸菌29%组显著高于37%组和41%组(P<0.05),大肠杆菌41%组显著低于29%和33%组(P<0.05);盲肠pH值升高;腹泻率和死亡率降低。日粮NDF水平提高对断奶獭兔的肠道健康有利,NDF 41%组断奶獭兔肠道最健康。This research evaluated the effect of dietary neutral detergent fiber （NDF） level on blood immune cells and intestinal characteristics of Rex rabbits at weaning in order to define the relationship between dietary NDF and intestinal health, and provide a theoretical basis for diet formulation for Rex rabbits at weaning. Fif- ty-six newly weaned rabbits （35 d） were randomly assigned into 4 groups （14 rabbits per group with each rab- bit considered a replicate for statistical purposes）, and the groups received different diets with NDF levels of 29%, 33%, 37% and41%, respectively. The experiment comprised a 5 d pre-trial period and a 28 d experi- ment period. Diarrhea rate and mortality were recorded during the entire trial. At the end of the trial, 6 rabbits from each group were slaughtered for sampling of blood and intestinal indicators. It was found that with in- creasing dietary NDF, the count of white blood cells did not differ between treatments （P〉0.05）, while the lymphocyte count was significantly increased （P〈0.05）. sIgA＋ cell counts in the duodenum and jejunum were increased with dietary NDF level （P^0.05）, but this trend was not observed in the ileum （P〉0.05）. Moreover, the total number of intestinal bacteria was similar for the 4 treatments （P〉0.05）. Bacteroides was increased at lower MDF with the 33% NDF group being significantly higher than the other groups （P〈0.05）. Cecum Lactobacillus and Escherichia coli numbers generally decreased with increasing dietary NDF level： spe- cifically, the 29 % NDF group got the highest Lactobacillus count while 41% NDF group had the lowest E. coli number （P〈0.05）. Furthermore, cecum pH was increased with dietary NDF level while diarrhea rate and mortality were decreased. In summary, among tested treatments, the 41% dietary NDF level optimized the in- testinal health of Rex rabbits at weaning.

关 键 词：獭兔 中性洗涤纤维 免疫球蛋白 肠道环境 

分 类 号：S829.1[农业科学—畜牧学]