Sidt2基因剔除小鼠的肺部形态学改变  

Lung morphology change in Sidt2 knockout mice

作　　者：蒋雪[1,2] 章尧[2,3] 王李卓[2,3] 王云[1] 夏礼斌 陈月平[4] 王莹[1] 高家林[4] JIANG Xue ZHANG Yao WANG Lizhuo WANG Yun XIA Libing CHEN Yueping WANG Yin GAO Jialing(Department of Respiratory Medicine ,The First Affiliated Hospital of Wannan Medical College, Wuhu 241001, China)

机构地区：[1]皖南医学院第一附属医院弋矶山医院呼吸内科,安徽芜湖241001 [2]皖南医学院活性生物大分子安徽省重点实验室,安徽芜湖241002 [3]皖南医学院生物化学教研室,安徽芜湖241002 [4]皖南医学院第一附属医院弋矶山医院内分泌科,安徽芜湖241001

出　　处：《皖南医学院学报》2016年第4期307-310,共4页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(81200632;81471002);安徽省自然科学基金项目(1308085QH134);弋矶山医院引进人才基金项目(2011014)

摘　　要：目的:观察Sidt2基因剔除小鼠的肺组织超微结构,探讨Sidt2基因在肺脏形态发育过程中的作用。方法:将利用基因打靶及同源重组技术获得的Sidt2基因全身剔除小鼠和野生型B129品系小鼠合笼交配,鼠尾提取DNA进行基因型鉴定,挑选出子代Sidt2-/+小鼠,同笼交配,再挑选出子代Sidt2-/-小鼠为实验组,以同窝野生型小鼠为对照,进行DNA、RNA和Western鉴定,并行肺组织光镜和电镜形态学观察。结果:通过DNA、RNA、蛋白质水平验证模型构造成功。光镜下对照鼠肺泡壁光滑,仅有少量粒细胞浸润;模型鼠肺泡间质内毛细血管扩张、充血,多处炎症细胞呈灶状分布。电镜下对照鼠的肺组织形态较规则;模型鼠可见肺泡上皮细胞崩解,毛细血管充血及基底膜增厚。结论:Sidt2基因剔除可影响小鼠肺组织形态,表现为细胞坏死,毛细血管充血及基底膜增厚,大量炎症细胞浸润,造成肺损伤。Objective： To investigate the role of Sidt2 gene in the lung morphogenesis through examining the lung tissue ultrastructure in Sidt2 knockout mice.Methods： Sidt2（- /-） mice generated by gene targeting and homologous recombination technology were mated with wild-type B129 mice,the genotype was identified by DNA extracted from rat tail.The offspring with the genotype Sidt2（- / ＋） were crossed each other,the Sidt2（- /-） mice in the F3 generation were selected as experimental group,wild-type littermates as control mice.Lung tissue morphology was observed by light microscopy and electron microscopy after confirming genotype with DNA,RNA and Western blotting. Results： Animal models were successfully established through verification by DNA,RNA and protein levels.Light microscopy indicated smooth alveolar wall of control mice,with a small amount of granulocyte infiltration.Dilated blood capillary,hyperemia,and focally distributed inflammatory cells were observed in alveolar interstitial of model mice.Lung morphology in control mice viewed by electron microscopy,while alveolar epithelial cells disruption,capillary hyperemia and basement membrane thickening were shown in experimental mice.Conclusion： Sidt2 knockout can affect the lung morphology,characterized by cell apoptosis,capillary hyperemia and basement membrane thickening as well as a large number of inflammatory cells infiltration,thus leading to lung damage.

关 键 词：溶酶体膜蛋白 Sidt2 肺部形态 炎症 肺损伤 

分 类 号：Q786[生物学—分子生物学] R563[医药卫生—呼吸系统]