巨噬细胞诱导极化对痛风炎症反应的影响  

Effects of differentiated macrophages on gouty inflammation

作　　者：张梦莹[1] 李志[2] 李雪琴[1] 钟民[1] 吕坤[1] ZHANG Mengying LI Zhi LI Xueqin ZHONG Min LYU Kun(Central Laboratory ,The First Affiliated Hospital of Wannan Medical College ,Wuhu 241001, China)

机构地区：[1]皖南医学院第一附属医院弋矶山医院中心实验室,安徽芜湖241001 [2]皖南医学院第一附属医院弋矶山医院风湿免疫科,安徽芜湖241001

出　　处：《皖南医学院学报》2016年第4期320-323,共4页Journal of Wannan Medical College

基　　金：皖南医学院中青年科研基金项目(WK2013F07)

摘　　要：目的:了解体外诱导极化的M1和M2型巨噬细胞对尿酸钠结晶(MSU)诱导的急性痛风模型炎症反应的影响。方法:选用雄性BALB/c小鼠作为实验对象,随机分为空白对照组、模型组、模型M1型巨噬细胞组和模型M2型巨噬细胞组。小鼠皮下气囊注射MSU结晶诱导小鼠急性痛风模型。体外将巨噬细胞诱导极化为M1和M2型巨噬细胞,并分别注射于小鼠痛风模型皮下气囊。于炎症诱导后4 h、12 h和24 h检测不同组别皮下气囊浸润的炎性细胞数量变化,并应用ELISA法测定囊内灌洗液中IL-1β、TGF-β的水平。结果:1在4 h、12 h和24 h时间点模型组、模型M1型巨噬细胞组和模型M2型巨噬细胞组皮下气囊炎性细胞均高于空白对照组(P<0.01)。在4 h时间点,模型组及模型M1型巨噬细胞组和模型M2型巨噬细胞组间皮下气囊炎性细胞数量无差异(P>0.05);而在12 h和24 h,模型M1型巨噬细胞组皮下气囊炎性细胞高于模型组,而模型M2型巨噬细胞组皮下气囊炎性细胞低于模型组(P<0.05)。2在3个时间点,模型组、模型M1型巨噬细胞组及模型M2型巨噬细胞组IL-1β均高于空白对照组(P<0.01);在3个时间点比较,IL-1β水平在模型组低于模型M1型巨噬细胞组(P<0.01),而高于模型M2型巨噬细胞组(P<0.01)。TGF-β水平在模型组高于模型M1型巨噬细胞组(P<0.01),而低于模型M2型巨噬细胞组(P<0.01)。结论:巨噬细胞的极化在痛风炎症反应不同阶段起到不同的作用。Objective： To observe the effects of polarized M1 and M2 macrophages in vitro on monosodium urate（ MSU） crystals-induced acute gouty inflammation.Methods： Male BALB / c mice,selected as experimental models,were randomly divided into group of blank control,models,M1 macrophages and M2 macrophages.Acute mouse gouty inflammation model was developed by subcutaneous injection of MSU crystals.M1 and M2 macrophages were polarized in vitro and injected into subcutaneous air sacs of gouty inflammation model.The levels of IL-1β and TGF-β in subcutaneous air sacs were dynamicaly detected by ELISA before and after infusion of macrophages.Results： 1The number of infiltrate inflammatory cells from model group,M1 macrophage group and M2 macrophage group was significantly increased compared to the blank controls at 4 h,12 h,and 24 h（ P〈0.01）,whereas remained no difference at 4 h among groups of models,M1 macrophages an M2 macrophages（ P〉0.05）.However,the number of inflammatory cells of model group was significant lower than that of M1 macrophages group,but significant higher than that of M2 macrophages group at 12 h and 24 h after stimulation（ P〈0.05）; 2IL-1β level in model group and groups of M1 macrophages and M2 macrophages was higher than that of control group at 3 time points（ P〈0.01）. IL- 1βlevel in model group was lower than that of M2 macrophages group,yet higher than that of M1 macrophages group（ P〈0.01）.Model group had higher TGF-β level than M1 macrophages group,but lower level than M2 macrophages group.Conclusion： Differentiation of macrophages may diversely function in the conversion of inflammation of the gout.

关 键 词：巨噬细胞 尿酸钠结晶 痛风 IL-1Β TGF-Β 

分 类 号：R589.7[医药卫生—内分泌]