金纳米棒-聚苯胺核壳纳米材料的光学相干层析成像能力研究  

A Study of Gold Nanorod-polyaniline Core-shell Nanoparticles for Enhanced Optical Coherence Tomography Imaging

作　　者：刘智明[1] 於剑锋 郭周义[1] LIU Zhiming YU Jianfeng GUO Zhouyi(MOE Key Laboratory of Laser Life Science ＆ SATCM Third Grade Laboratory of Chinese Medicine and Photonics Technology, College of Biophotonics, South China Normal University, Guangzhou 510631, Guangdong, China)

机构地区：[1]华南师范大学生物光子学研究院,激光生命科学教育部重点实验室和中医药与光子技术国家中医药管理局三级实验室,广东广州510631

出　　处：《激光生物学报》2016年第4期315-318,341,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金(No.61275187,No.61335011,No.21505047);广东省自然科学基金重点项目(No.2014A030311024);广东省自然科学基金博士点启动基金(No.2014A030310306);教育部高等学校博士学科点专项科研基金(No.20114407110001&No.20134407120003);广东省重大科技专项基金(No.2012A080203008);广东省教育部产学研结合重点项目(No.2011A090200011);广东省教育厅科技创新项目(No.2013KJCX0052);广东省科技计划项目(No.2014A020212282);华南师范大学青年教师科研培育基金(No.14KJ10)

摘　　要：对比试剂的使用能够显著提升光学相干层析(OCT)的成像效果。聚苯胺(PANI)是一种有机导电聚合物,在近红外(NIR)区有着很强的光吸收。本文采用PANI对常见的OCT成像对比试剂--金纳米棒(GNRs)进行修饰,合成了PANI/GNRs核壳粒子,并对其OCT成像对比能力进行了研究。PANI/GNRs展现出良好的NIR光吸收特性;同时,PANI对GNRs的包裹也显著提升了金纳米结构的稳定性、降低了GNRs原有的毒性。选用离体猪肝组织作为检测样本,发现纳米材料使用能够显著提升OCT的成像效果。与未修饰的GNRs及PANI粒子相比,PANI/GNRs的OCT成像对比效果明显更好。因此,PANI包裹的GNRs核壳纳米材料有望成为一种低毒性且效果良好的OCT对比试剂用于生物组织成像。Optical coherence tomography （OCT）imaging using contrast agent is a useful technique to improve the con-trast effect.Polyaniline （PANI）nanoparticles,an organic conducting polymer,exhibit strong near-infrared absorbance. Herein,we adopted PANI as the shell material for coating gold nanorods （GNRs）.To investigate the contrast effect of PANI-functionalized GNRs,we have synthesized three different kinds of nanoparticles containing polyaniline （PANI）nanopartcles,GNRs and PANI /GNRs nanocomposites.They were used as potential OCT contrast agents due to their similar optical properties that they exhibit strong light absorption in near-infrared region.OCT imaging in ex vivo pig liver tissues with the three kinds of nanoparticles was studied.The experimental results indicated that the penetration depth of the signals from pig liver tissues with nanoparticles was significantly lower than that from pig liver tissues without nanop-articles.Moreover,in comparison to the bare GNRs,PANI functionalized GNRs exhibited better OCT contrast effect.

关 键 词：光学相干层析成像 对比试剂 聚苯胺 金纳米棒 

分 类 号：TB852.1[一般工业技术—摄影技术]