RGD/FA双靶纳米金棒的制备及其在肿瘤细胞协同靶向成像与光热治疗中的应用  

Preparation and Synergistic Targeted Imaging and Hyperthermia on Tumor Cell Using Dual Targeting Gold Nanorods with RGD / FA

作　　者：宗智慧[1] 刘刚[2] 汪静[2] ZONG Zhihui LIU Gang WANG Jing(BengBu Medical College, Bengbu 233030, Anhui, China College of Pharmacy, Ningxia Medical University , Ningxia Collaborative Innovation Center of Modern Hui Medicine , Key Laboratory of Hui Ethnic Medicine Modernization, Chinese Ministry of Education at Ningxia Medical University , Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia,China)

机构地区：[1]蚌埠医学院,安徽蚌埠233030 [2]宁夏医科大学药学院,宁夏回医药协同创新中心,回医药现代化省部共建教育部重点实验室,宁夏回药现代化工程技术中心,宁夏银川750004

出　　处：《激光生物学报》2016年第4期319-324,共6页Acta Laser Biology Sinica

基　　金：宁夏自然科学基金(NZ14083)

摘　　要：目的:通过制备RGD/FA双靶纳米金考察其与高表达整合素与叶酸受体B16细胞的协同靶向成像与热疗作用;方法:采用功能化PEG分子将靶向小分子RGD与叶酸通过强健Au-S键连接至纳米金棒表面,利用激光共聚焦与808 nm近红外激光器评价修饰纳米金的协同靶向作用;结果:RGD与叶酸分子被成功连接于纳米金表面,且双靶纳米金对小鼠黑色素瘤细胞具有较好的协同靶向作用;结论:同时靶向同一肿瘤细胞的不同表位,可克服单一靶向功能化纳米粒子难以在肿瘤位点有效积累的问题,本研究为多功能纳米金棒在临床肿瘤早期诊断与光热治疗中的应用提供研究基础。Objective：The synergistic targeted imaging and hyperthermia on B16 cell with overexpression of integrin and folate receptor were investigated through preparation of dual gold nanorods with RGD and folic acid;Method：The functional PEG with targeted small molecule RGD and FA was applied through strong Au-S bond connection to the sur-face of gold nanorods.Synergistic targeting of dual targeted gold nanorods on B16 was evaluated through laser scanning confocal fluorescence microscope and NIR-808 nm laser;Result：RGD and FA were connected successfully to the sur-face of gold nanorods,and dual targeting gold nanorods played a role on synergistic targeting to B16 cells;Conclusion：Targeting different cell epitopes of same cell simultaneously can overcome noneffective accumulation of single targeting gold nanorods on tumor sites.The study can support the research basis in clinical tumor early diagnosis and hyperthermia of gold nanorods.

关 键 词：纳米金棒 RGD/FA 功能化PEG 协同靶向 

分 类 号：Q6-33[生物学—生物物理学]