原子力显微镜研究多西他赛对人肺腺癌A549细胞的作用机制  

A Study on the Mechanics of Docetaxel on Lung Cancer Cells of A549 Using AFM

作　　者：黄雯[1,2] 王美霞[3] 陈伊琳[2,4] 刘绍静[2,4] 盛良翮[5] 徐萌[5] 朱德斌[1] 邢晓波[1,2] HUANG Wen WANG Meixia CHEN Yilin LIU Shaojing SHENG Lianghe XU Meng ZHU Debin XING Xiaobo(MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, Guangdong, China South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, Guangdong, China Guangdong Prevention and Treatment Center for Occupational Diseases, Guangzhou 510300, Guangdong, China School of Physics and Telecommunication Engineering, South China Normal University, Guangzhou 510006, Guangdong, China Oncology Department of the First Affiliated Hospital, Jinan University, Guangzhou 510632, Guangdong, China)

机构地区：[1]华南师范大学生物光子学研究院激光生命科学研究所教育部重点实验室,广东广州510631 [2]华南师范大学华南先进光电子研究院,广东广州510006 [3]广东省职业病防治院职业卫生评价所,广东广州510300 [4]华南师范大学物理与电信工程学院,广东广州510006 [5]暨南大学附属第一医院肿瘤科,广东广州510632

出　　处：《激光生物学报》2016年第4期325-330,349,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(61177077;81371877);广东省自然科学基金项目(2013B090500123;2014A030313432;2016A020221030;2013B090500034);广东省创新团队项目(201001D0104799318)

摘　　要：利用原子力显微镜(AFM)研究了化疗药物多西他赛对肺腺癌细胞A549的作用机制。选取了不同浓度多西他赛作用下的A549细胞,观察和分析了多西他赛对A549细胞形貌信息和生物力学特性的影响。结果表明细胞的峰谷差和超微结构的平均粗糙度随着药物浓度增加而减小,表面粘附力随着药物浓度增加而增大。该结果对AFM可作为一种利用纳米量级分辨率进行药物药效评估的工具进行了佐证。The mechanics of docetaxel on lung cancer cells of A549 has been studied by atomic force microcopy （AFM）.Different cells of A549 affected by the different concentrations of docetaxel were used.The analysis showed that docetaxel can affect the morphological information and biomechanical properties.The results showed that the value of valley-to-peak and average roughness decrease,and adhesive force of cell surface increases when the concentration of do-cetaxel increases.The AFM may potentially be developed into a tool in evaluating drug effect at nanometer scale is sup-ported.

关 键 词：原子力显微镜 多西他赛 A549细胞 

分 类 号：R318.51[医药卫生—生物医学工程]