乙肝五项、HBV-DNA定量及乙肝前S1抗原联合检测用于诊断乙肝的临床价值分析  

The Analysis of Diagnostic Value of Combined Pre-S1 Antigen and HBV-DNA and Multi-5 Test Panel in Hepatitis B Detection

作　　者：刘佩[1] 赵旭鸿[1] 

机构地区：[1]上海市杨浦区中心医院检验科,上海200090

出　　处：《标记免疫分析与临床》2016年第11期1275-1278,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的分析乙肝五项与病毒(HBV)前S1抗原及病毒DNA的相关性,并探讨联合检测对乙肝诊断的临床价值。方法选取本院门诊部和住院部2013年10月至2015年10月期间收治的患者作为研究对象,收集其HBsAg阳性血清,采用酶联免疫吸附法(ELISA)检测其前S1抗原和乙肝五项,采用荧光定量PCR法检测其HBV-DNA水平,对比分析不同模式下前S1抗原的表达水平和HBV-DNA的水平,并分析其相关性。结果乙肝前S1抗原阳性率与HBV-DNA阳性率在不同HBV-M模式下比较,差异无统计学意义(P>0.05)。此外1+3+5+组中HBV-DNA阳性拷贝数较高,多数高于105copies/m L;而1+4+5+、1+2+、1+3+、1+4+和1+5+组的前S1+与前S1-组相比,其HBV-DNA阳性率差异无统计学意义(P>0.05)。HBsAg阳性血清标本中前S1抗原在HBe Ag+组中阳性率明显高于HBe Ag-组,差异具有统计学意义(P<0.05),HBsAg阳性血清标本中前S1抗原与HBe Ag具有一定的相关性。HBsAg阳性血清标本中前S1抗原在HBV-DNA+组中阳性率明显高于在HBV-DNA-组,差异具有统计学意义(P<0.05),HBsAg阳性血清标本中前S1抗原与HBV-DNA具有一定的相关性。结论前S1抗原可较好反映乙肝病毒存在和复制的情况,将前S1与乙肝五项和HBV-DNA进行联合检测可较早发现较低水平病毒的感染,其可作为抗病毒疗效的评判指标,对乙肝的临床诊断和疗效评价均具有较好的应用价值。Objective To analyze multi-5 test panel of hepatitis b virus（ HBV）,pre-S1 antigen and the correlation of the viral DNA,and to explore the clinical value of diagnosis of combined detection of hepatitis B. Methods We selected the outpatients and inpatients in our hospital during October 2013 to October 2015 as the research objects. Collected HBsAg positive serum samples were used for enzyme-linked immunosorbent assay（ ELISA） to detect pre-S1 and multi-5 test panel and fluorescent quantitative PCR method to detect the HBV-DNA. The comparative analysis of different conditions of pre-S1 antigen and HBV-DNA content was conducted and their correlation was analyzed. Results When the positive rates of pre-S1 antigen and HBV-DNA in different HBV-Mmode were compared,the differences were not significant（ P〉 0. 05）. The positive rate of pre-S1 antigen in HBe Ag positive group was higher than that of control group,the difference was statistically significant（ P〈 0. 05）. In HBsAg positive serum samples,pre-S1 and HBe Ag were correlated. The positive rate of Pre-S1 antigen in HBV-DNA group was higher than control group,the difference was statistically significant（ P〈 0. 05）. In HBsAg positive serum samples,Pre-S1 antigen and HBV-DNA were correlated. Conclusion Pre-S1 antigen can better reflect the existence and hepatitis B virus replication. The combination detection of pre-S1,multi-5 test panel and HBV-DNA for HBV can detect early virus infections. It can be used as antiviral efficacy indicator. It might have applicable value in the clinical diagnosis and treatment of Hepatitis B infection.

关 键 词：乙肝两对半 DNA 前S1抗原 乙肝病毒 临床诊断 

分 类 号：R512.62[医药卫生—内科学]