Study on the Bacteriostatic Effects of 7 Kinds of Chinese Herbal Medicines such as Ophiopogon japonicus and Comb  

麦冬、蜂巢等7味中草药抑菌作用研究（英文）

作　　者：王玮[1] 姜海烽 邬智伟 钱炯[1] 汪小迪 许锦秀[2] 付浩[2] 傅兰英[3] 

机构地区：[1]新乡医学院三全学院临床医学院,河南新乡453003 [2]新乡医学院三全学院医疗技术学院,河南新乡453003 [3]郑州升达经贸管理学院体育系,河南郑州451191

出　　处：《Agricultural Science & Technology》2016年第11期2560-2563,共4页农业科学与技术（英文版）

基　　金：新乡医学院三全学院大学生自然科学研究重点项目,5种中草药体外抑菌活性筛选(2015002zz)

摘　　要：[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, and Allium macrostemon. [Method] Herbal active ingredients were extracted by water boiling method and a bacteriostatic experiment was per- formed by oxford cup diffusion method, to select Chinese herbs with antibacterial effects. MIC values of seven kinds of Chinese herbal medicine were determined by in vitro half-fold dilution method. [Results] Root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, Allium macrostemon, Juncus effusus and Xanthi- um sibiricum had bacteriostatic effects on Escherichia coil and Staphylococcus au- reus in varying degrees. Xanthium and leaf of Ophiopogon japonicus performed bet- ter on Escherichia coil, with MIC values at 7.81 and 15.63 mg/ml, respectively. Panax pseudoginseng and Xanthium performed better on Staphylococcus aureus with MIC values at 15.63 and 31.25 mg/ml. And the drug＇s effect was positively correlated with the concentration. [Conclusion] Xanthium sibiricum is the best bacte- riostatic effect, followed by leaf of Ophiopogon japonicus, and Juncus effusus per- forms the poorest. Panax pseudoginseng showed the best bacteriostatic effect, fol- lowed by Xanthium sibiricum and Juncus effusus performs the poorest. These pro- vide references for clinic safety.[目的]探究麦冬根、麦冬叶、蜂巢、三七、薤白等7味中草药对大肠杆菌、金黄色葡萄球菌的单方抑菌效果。[方法]使用水煮法分别提取中草药有效成分,并用牛津杯扩散法进行抑菌实验,筛选出有较强抑菌效果的中草药。通过试管倍半稀释法测定7种中草药的MIC值。[结果]麦冬根、麦冬叶、蜂巢、三七、薤白、灯芯草、苍耳子均对大肠杆菌、金黄色葡萄球菌有不同程度的抑菌作用,其中苍耳子和麦冬叶对大肠杆菌有较好的抑菌效果,MIC为7.81和15.63 mg/ml。三七和苍耳子对金黄色葡萄球菌的抑制效果明显MIC分别为15.63和31.25 mg/ml。且药物的抑菌效果与其浓度呈正相关。[结论]苍耳子对大肠杆菌的抑菌效果最强,麦冬叶次之,灯芯草最弱。三七对金黄葡萄球菌的抑菌效果最强,苍耳子次之,灯芯草最弱,为其临床安全使用提供参考。

关 键 词：Ophiopogon japonicus HONEYCOMB Panax pseudoginseng Allium macrostemon Juncus effusus Xanthium sibiricum Bacteriostatic effects 

分 类 号：R285[医药卫生—中药学]