谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响  

Effects of Glutamate on Intestinal Energy Metabolism in the Lipopolysaccharide-Challenged Weaned Piglets

作　　者：秦琴[1] 王秀英[1] 吴欢听 朱惠玲[1] 刘玉兰[1] 

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点实验室,武汉430023

出　　处：《动物营养学报》2016年第11期3618-3625,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：湖北省教育厅优秀中青年科技创新团队项目(T201508)

摘　　要：本试验旨在研究谷氨酸(Glu)对脂多糖(LPS)刺激断奶仔猪肠道能量代谢的影响。选择24头断奶仔猪分为4个组,分别为对照组、LPS组、LPS+1.0%Glu组和LPS+2.0%Glu组,每组6个重复,每个重复1头猪。于试验第28天,试验组猪注射100μg/kg BW LPS,对照组注射等量的生理盐水,4 h后屠宰,取肠道样品待测。结果表明:1)与对照组相比,LPS刺激导致断奶仔猪空肠三磷酸腺苷(ATP)、腺苷酸池(TAN)含量和能荷(EC)显著降低(P<0.05),一磷酸腺苷(AMP)/ATP值显著升高(P<0.05);与LPS组相比,LPS+2.0%Glu组显著提高了空肠ATP、二磷酸腺苷(ADP)和TAN含量(P<0.05)。2)与对照组相比,LPS刺激导致断奶仔猪回肠柠檬酸合成酶和α-酮戊二酸脱氢酶系活性极显著降低(P<0.01),空肠α-酮戊二酸脱氢酶系活性有降低趋势(P=0.092);与LPS组相比,除LPS+1.0%Glu组回肠柠檬酸合成酶活性显著降低(P<0.05)外,Glu对空肠和回肠三羧酸循环关键酶活性无显著影响(P>0.05)。3)与对照组相比,LPS刺激导致空肠过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC1α)及回肠沉默信号调控因子1(Sirt1)和PGC1α的mRNA表达量极显著降低(P<0.01);与LPS组相比,LPS+2.0%Glu组有提高空肠PGC1α(P=0.067)和回肠Sirt1(P=0.053)mRNA表达量的趋势,LPS+1.0%Glu组有提高回肠Sirt1 mRNA表达量的趋势(P=0.070)。由此可见,Glu可以改善LPS刺激导致的肠道能量损耗状态。This study was aimed to investigate the effects of glutamate（ Glu） on intestinal energy metabolism in the lipopolysaccharide（ LPS）-challenged weaned piglets. Twenty-four weaned pigs were assigned to four groups as control group,LPS group,LPS ＋ 1. 0 % Glu group and LPS ＋ 2. 0 % Glu group with 6 replicates each and 1 pig in per replicate. On the 28 thday of the trial,the piglets in the experimental groups were injected with 100 μg / kg BWLPS,and the piglets in the control group were injected with the same amount of 0. 9 %Na Cl solution. At 4 h post-injection,pigs were slaughtered and intestinal samples were collected for further analysis. The results showed as follows： 1） LPS challenge significantly decreased ATP and total adenine nucleotide（ TAN） contents and energy charge（ P〈0. 05）,but significantly increased the ratio of AMP to ATP（ P〈0. 05） in jejunum compared with the control group; LPS ＋ 2. 0 % Glu group significantly increased the contents of ATP,ADP and TAN（ P〈0. 05） in jejunum compared with LPS group. 2） Compared with the control group,LPS challenge had a tendency to decrease the alpha-oxoglutarate dehydrogenase complex activity（ P = 0. 092） in jejunum and extremely significantly decreased the activities of citrate synthase and alpha-oxoglutarate dehydrogenase complex（ P〈0. 01） in ileum; compared with LPS group,Glu had no significant effects on the activities of tricarboxylic acid cycle key enzymes（ P〉0. 05） in jejunum and ileum,except for the citrate synthase activity in ileum was significantly reduced in LPS ＋ 1. 0 % Glu group（ P〈0. 05）. 3） LPS challenge extremely significantly decreased the mRNA expressions of peroxisome proliferator-activated receptor gamma coactivator-1 α（ PGC1 α） in jejunum and silent information regulator 1（ Sirt1） and PGC1 α in ileum（ P〈0. 01） compared with the control group; compared with LPS group,LPS ＋ 2. 0% Glu group had a tendency to increase the mRNA expressions of PGC1 α in jejunum（ P

关 键 词：谷氨酸 脂多糖 断奶仔猪 肠道 能量代谢 

分 类 号：S828[农业科学—畜牧学]