稀土元素镧对丹参活性物质积累及其合成途径中酶基因表达的影响  

Effect of Lanthanum on accumulation of active constituent and key enzymes expression of Salvia miltiorrhiza hairy root

作　　者：边丽华[1,2] 邹琳[1] 周冰谦[1] 刘伟[1] 周洁[1,3] 王晓[1] 

机构地区：[1]山东省中药质量控制技术重点实验室山东省分析测试中心,山东济南250014 [2]山东中医药大学,山东济南250355 [3]济南大学生物科学与技术学院,山东济南250012

出　　处：《中国中药杂志》2016年第23期4344-4349,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81303161);山东省自然科学基金项目(ZR2013HQ037);山东省科技发展计划项目(2015GSF119021,2015GSF119026);中央本级重大增减支项目(2060302-1601-19);山东省科学院先导科技专项

摘　　要：该文研究了镧对丹参毛状根中活性物质积累及其合成途径中酶基因表达的影响,为揭示土壤因子镧影响丹参药材品质形成的环境机制奠定基础。采用HPLC测定丹参毛状根中活性物质含量;采用Tiangen多糖多酚植物总RNA提取试剂盒提取RNA,采用Takara反转录试剂盒合成c DNA,采用PCR荧光定量试剂盒进行实时定量分析,采用SPSS软件进行数据分析。结果显示镧处理对丹参毛状根内丹参酮类和酚酸类的积累表现出显著的促进效应,处理后9 d其酚酸类成分含量达到峰值,丹参酮类成分含量随处理时间的延长而增加(15 d内);镧处理后丹参体内活性物质积累的峰值时间相对滞后于酶基因表达的峰值时期,FPPS,TAT,HPPR 3个酶基因与丹参酮ⅡA、迷迭香酸和丹酚酸B含量变化趋势相似,暗示FPPS,TAT,HPPR 3个酶基因在镧诱导的丹参活性物质积累中发挥重要作用。The effect of Lanthanum on the accumulation of active constituent and key enzymes expression of Salvia miltiorrhiza hairy root were studied and furthermore signaling molecules mediating the synthesis of secondary metabolism was also defined in order to provide references for the reveal of synthesis mechanism of active constituent of S. miltiorrhiza hairy root inducing by Lanthanum. The content of active constituents were detected by HPLC. RNA was extracted with RNA prep Pure RNA purification kit（ Tiangen）. The results shows that La Cl3 processing promoted the accumulation of tanshinones and phenolic acids in S. miltiorrhiza hairy root. The accumulation of phenolic acids reached the highest at 9 d after treatment,and tanshinones accumulation continued to increase in 15 days.Accumulation of active substance in S. miltiorrhiza may relate with FPPS,TAT,HPPR several key enzyme activation.

关 键 词：丹参 毛状根 活性物质 关键酶基因 

分 类 号：S567.53[农业科学—中草药栽培]