傣药望江南中的一个新的降倍半萜类化合物及其活性研究  

A new nor-sesquiterpene from Cassia occidentalis and its bioactivity

作　　者：秦瑞欣 左茜[1] 黄萧涵 马航赢 杨艳[2] 邢欢欢[2] 周玲[2] 周敏[2] 胡秋芬[2] 

机构地区：[1]云南师范大学附属中学,云南昆明650106 [2]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650106

出　　处：《中国中药杂志》2016年第23期4389-4392,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(21362044;21032085);云南省高校科技创新团队项目(IRTSTYN 2014-11)

摘　　要：对傣药望江南Cassia occidentalis的化学成分进行研究,望江南细枝采用乙醇提取,然后用乙酸乙酯萃取,乙酸乙酯萃取物用硅胶柱色谱及PR-HPLC制备色谱技术进行多次分离,得到1个降倍半萜3-异丙基-1,6-二甲氧基-5-甲基萘-7-醇1和一个倍半萜2,7-二羟基-4-异丙基-6-甲基萘-1-甲醛2,其结构通过谱学数据鉴定。化合物1为新化合物,化合物2也首次从望江南中分离得到。并用MTT法测定了化合物1对NB4,A549,SHSY5Y,PC3,MCF7 5株人癌细胞株的细胞毒活性,其IC50分别为(1.8±0.2),(1.2±0.2),(0.9±0.1),(2.2±0.3),(2.6±0.3)μmol·L-1。The phytochemistry investigation on the Cassia occidentalis,a Dai Medicine,was carried out. The C. occidentalis was extracted with ethanol and then partitioned with Et OAc. The Et OAc soluble materials were subjected repeatedly to column chromatography on silica gel and preparative RP-HPLC,leading to isolation of a nor-sesquiterpene,3-isopropyl-1,6-dimethoxy-5-methyl-naphthalen-7-ol（ 1）,and a sesquiterpene,2,7-dihydroxy-4-isopropyl-6-methyl-naphthalene-1-carbaldehyde（ 2）. Their structures were determined by means of spectroscopic studies. Compound 1 is a new compound. Compound 2 is also isolated from C. occidentalis for the first time. In addition,the cytotoxicity of compound 1 for NB4,A549,SHSY5 Y,PC3,and MCF7 cells line was assayed by using the MTT method,and it displayed potential cytotoxicity for the tested cancer cell-line with IC50 valves of（ 1. 8 ± 0. 2）,（ 1. 2 ± 0. 2）,（ 0. 9 ± 0. 1）,（ 2. 2 ± 0. 3）,（ 2. 6 ± 0. 3） μmol·L-1,respectively.

关 键 词：望江南 降倍半萜 细胞毒活性 

分 类 号：R29[医药卫生—民族医学]