NtMYC1a转录因子的克隆与功能初步分析  

Cloning and Function Analysis of Transcription Factor NtMYC1a from Tobacco

作　　者：郭红祥[1] 李富欣[2] 刘巧真[3] 李素敏[1] 郭爱芳 李斐[1] 丁超[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]河南省烟草公司济源市公司,河南济源454650 [3]河南省农业科学院烟草研究中心,河南许昌461000 [4]河南省获嘉县种子公司,河南获嘉453822

出　　处：《江西农业学报》2016年第12期80-82,86,共4页Acta Agriculturae Jiangxi

基　　金：河南省烟草公司科技重点项目(HYKJ201308)

摘　　要：从烟草根系中克隆NtMYC1a基因,构建表达载体并在烟草中瞬时表达,探讨了NtMYC1a转录因子在烟碱生物合成中的作用。转基因烟草中的NtMYC1a表达显著升高,表明成功构建了NtMYC1a表达载体并转化了烟草;转基因烟草中的PMT表达量显著升高,表明NtMYC1a能够正调控烟碱的合成;茉莉酸与干旱处理烟草后,检测到NtMYC1a和PMT的表达量显著升高,表明在茉莉酸、干旱促进烟碱合成的生物学过程中,NtMYC1a具有正调控的功能。The gene NtMYC1α in the roots of tobacco was cloned, its expression vector was constructed and transiently ex- pressed in tobacco, and the role of transcription factor NtMYC1α in the biosynthesis of nicotine was investigated. The expression level of NtMYCla in transgenic tobacco plants was notably increased, suggesting that the constructed expression vector with Nt- MYC1α had been successfully transformed into tobacco plants. The expression level of PMT in transgenic tobacco plants was re- markably enhanced, indicating that NtMYCIa could positively regulate the biosynthesis of nicotine. After tobacco plants were trea- ted with jasmonic acid or drought, the expression levels of both NtMYCla and PMT in them were significantly increased, showing that NtMYC1α played a positive regulatory role in nicotine biosynthesis promoted by jasmonic acid or drought.

关 键 词：转录因子 NtMYC1a 烟碱 茉莉酸 干旱 

分 类 号：Q785[生物学—分子生物学]