不规则抗体与疑难配血相关性分析  

Analysis of correlation between irregular antibody screening and crossmatching incompatibility

作　　者：温会燕[1] 杜鸿[1] 林军[1] WEN Hui-yan DU Hong LIN Jun(Department of Blood Transfusion, the Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China)

机构地区：[1]苏州大学附属第二医院输血科,江苏苏州215004

出　　处：《中国卫生检验杂志》2016年第22期3320-3322,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的探寻输血患者不规则抗体的分布规律,分析疑难配血原因。方法回顾本院输血科自2013年7月-2015年6月住院拟输血患者不规则抗体情况,分析交叉配血中疑难配血的原因。结果在11 008例患者的不规则抗体筛查样本中阳性有208例,阳性率为1.89%。208例阳性样本检出特异性抗体占84.13%(175/208)、温自身抗体占6.73%(14/208)、冷凝集素占8.17%(17/208),其他占2.40%(5/208);5 944例交叉配血中疑难配血241例,占4.05%,其中患者存在特异性抗体占70.95%(171/241)、疑难血型占3.32%(8/241)、温自身抗体占5.81%(14/241)、冷凝集素占7.05%(17/241)、直抗阳性者占7.88%(19/241),献血者原因有含不规则抗体占1.66%(4/241)和样辫血抗凝不佳占0.41%(1/241)。结论输血前对患者进行不规则抗体筛查和鉴定,可预先了解患者体内血型抗体产生情况,同时也应注意疑难配血个体化原因的探寻。Objective To seek the distribution rule of irregular antibody in blood transfusion patients,and analyze the cause of crossmatching incompatibility. Methods Blood samples from department of blood transfusion in this hospital from July 2013 to June 2015 were tested through irregular antibody screening. The existence situation of irregular antibody of those samples is reviewed. The cause of crossmatching incompatibility is analyzed. Results Among the 11 008 samples tested through irregular antibody screening,208 cases（ 1. 89%） were positive. Among the 208 positive cases,175 cases belong to specific antibody,accounting for 84. 13%. Warm autoantibodies were found in 14 samples,accounting for 6. 73%. 17 cases were cold agglutinin,accounting for 8. 17%. 5 cases were positive for irregular antibody screening with non- specificity,accounting for 2. 40%. Among 5 944 crossmatching cases,241 cases belong to incompatibility,accounting for 4. 05%. Among 241 incompatibility cases,171 cases belong to specific antibody,accounting for 70. 95%. Difficult blood group was found in 8 cases,accounting for3. 32%. 14 cases were warm autoantibodies,accounting for 5. 81%. 17 cases were cold agglutinin,accounting for 7. 05%. Direct Coombs test showed positive result in 19 cases,accounting for 7. 88%. In addition,4 cases belong to irregular antibody from blood donors,accounting for 1. 66%. And 1 case was red cell agglutination in donor sample,accounting for 0. 41%. Conclusion Screening and analyzing for the presence of irregular antibody in patient blood before blood transfusion is important to understand the intracorporal situation of patients in advance. At the same time we should also pay attention to searching the cause of the individual crossmatching incompatibility.

关 键 词：不规则抗体 抗体筛查 抗体特异性鉴定 疑难配血 

分 类 号：R446.6[医药卫生—诊断学]