瘿花甲素对S180细胞抗肿瘤活性及其细胞周期的影响  

Effect of a diterpenoid compound rosthorin A on the antitumor activity and cell cycle of S180

作　　者：杨焕芝[1,2] 周轶平[2] 翁稚颖[2] 罗敏[2] 孙汉董[3] 李玛琳[2,4] YANG Huan-zhi ZHOU Yi-ping WENG Zhi-ying LUO Min SUN Han-dong LI Ma-lin(Department of Pharmacy, Kunming First People＇s Hospital, Kunming 650011 Yunnan Key Laboratory of Pharmacology for Natural Products, College of Pharmacy, Kunming Medical University, Kunming 650500 Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 College of Traditional Chinese Medicine, Yunan University of Traditional Chinese Medicine, Kunming 650500)

机构地区：[1]昆明市第一人民医院药学部,昆明650011 [2]昆明医科大学药学院暨云南省天然药物药理重点实验室,昆明650500 [3]中国科学院昆明植物研究所,昆明650204 [4]云南中医学院中药学院,昆明650500

出　　处：《中南药学》2016年第11期1202-1205,共4页Central South Pharmacy

基　　金：云南省自然科学基金重点项目子课题(No.2004C0008Z)

摘　　要：目的研究瘿花甲素（rosthorin A）体内外抗肿瘤活性及其作用机制。方法采用改良MTT法测试rosthorin A对S180细胞株增殖的影响;以荷S180肉瘤小鼠为模型,检测rosthorin A对荷瘤小鼠肉瘤重量、体重的影响;通过流式细胞术检测rosthorin A对S180细胞株和肉瘤组织细胞周期的影响。结果Rosthorin A对S180细胞株的IC50值为2.69 mg·L-1。小鼠经腹腔注射rosthorin A的LD50为83.20mg·kg-1。与溶剂对照组相比,rosthorin A在2-8 mg·kg-1内对荷S180肉瘤小鼠肿瘤生长无抑制作用（P〉0.05）,但可使S180细胞株和S180肉瘤组织细胞G0/G1期所占比例增多。结论 Rosthorin A对S180细胞株体外有较强的细胞毒活性,可通过阻滞细胞由G0/G1期进入S期抑制细胞增殖,体内在实验剂量范围内对荷S180肉瘤小鼠无抑瘤作用。Objective To determine the antitumor activity and mechanism of a diterpenoid compound rosthorin A from Isodon xerophilus. Methods Modified MTT assay was used to test the effect of rosthorin A on the proliferation of S180 tumor cell strain. The influence on tumor growth in mice with transplanted sarcoma（S180） was observed. The cell cycle of S180 cell lines and mouse sarcoma（S180） was analysed with Flow Cytometry. Results The IC50 of rosthorin A in S180 cell lines was 2.69 mg·L-1. Through intraperitoneal injection, the LD50 of rosthorin A in mice was 83.20 mg·kg-1. Compared with the solvent control group, there was no obvious difference（P 〉 0.05） in the inhibitory rate of tumor with rosthorin A at 2-8 mg·kg-1. But the cell cycle at G0/G1 phase of S180 was increased. Conclusion Rosthorin A has an obvious effect on inhibiting S180 cell proliferation in vitro and the mechanism might stop at the G0/G1 phase, but has no obvious antitumor activity for mouse transplanted sarcoma.

关 键 词：瘿花甲素 S180细胞 小鼠移植性肿瘤 细胞周期 抗肿瘤活性 

分 类 号：R285.5[医药卫生—中药学]