一种囊状幼虫病毒分子标记的克隆  

Cloning of a kind of molecular marker for Sacbrood virus

作　　者：熊翠玲[1] 林跃文 梁勤[1] 郑燕珍[1] 徐细建[1] 张曌南 黄枳腱 郭睿[1] 陈大福[1] Xiong Culling Lin Yuewenz Liang Qin Zheng Yanzhen Xu Xijian Zhang Zhaonan Huang Zhijian Guo Rui Chen Dafu(College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou 350002 Longtianyuewen Professional Corporation for Apicuhure, Quanzhou 362114)

机构地区：[1]福建农林大学蜂学学院,福州350002 [2]龙田跃文蜂业专业合作社,泉州362114

出　　处：《中国蜂业》2016年第12期14-16,21,共4页Apiculture of China

基　　金：国家蜂产业技术体系建设专项资金(CARS-45-KXJ7);福建农林大学科技发展资金(KF2015123);福建省自然科学基金指导性科技计划项目(2012D079)资助

摘　　要：蜜蜂囊状幼虫病是由囊状幼虫病毒(Sacbrood virus,SBV)侵染蜜蜂幼虫而导致的一种致死性病毒病。本研究根据Gene Bank上SBV多个毒株的基因组信息设计合成一对特异性引物,从出现囊状幼虫病典型症状的中蜂幼虫中扩增出一个大小约为106 bp的特异性SBV106片段,进而将其克隆进p GEM-T载体,经蓝白斑筛选出阳性质粒,Eco RⅠ酶切可得约106 bp大小的目的片段,阳性质粒原菌液测序结果显示该片段与SBV全序列(收录号:AF092924)的相似度达100%,证明该序列为SBV特有序列。上述结果表明SBV106片段可作为检测蜜蜂囊状幼虫病的分子标记,应用于养蜂生产。Sacbrood is a kind of fatal disease for honeybee larvae caused by SBV. In this study, a pair of primers was designed according to genome information of several SB~ strains published in GeneBank. A specific fragment about 106 bp was amplified from Apis cerana cerana larvae with obvious symptoms. Subsequently, this SBV106 fragment was cloned into pGEM-T vector, and after blue-white selection, positive plasmids were digested with EcoR I and a 106 bp fragment was obtained by agarose gel electrophoresis. The corresponding bacterial solution was sequenced and the result showed SBV106 has a 100% identity with SBV genome（GeneBank accession number： AF092924）. These results together suggested SBV106, as a molecular marker for detecting Sacbrood, could be applied to apicuhure.

关 键 词：囊状幼虫病毒 分子标记 PCR 克隆 

分 类 号：S895[农业科学—特种经济动物饲养]