紫背天葵的高效液相色谱法指纹图谱分析  

Analysis of high performance liquid chromatography Semiaquilegia fingerprint

作　　者：邵考珍 何淑莹 

机构地区：[1]佛山市南海区第七人民医院,广东528247

出　　处：《中国药物经济学》2016年第11期17-21,共5页China Journal of Pharmaceutical Economics

摘　　要：目的探讨紫背天葵的高效液相色谱法(HPLC)指纹图谱。方法采用色谱柱Shim-pack VP-ODS(250 mm×4.6 mm,5μm);以0.5%H3p O4(V/V)为流动相A,以50%乙腈为流动相B(V/V),进行梯度洗脱;流速为0.8 ml/min;柱温30℃;检测波长为280 nm;进样量10μl;运行时间为80 min。理论板数按矢车菊素-3-O-葡萄糖苷计算,不得低于3000。结果建立了紫背天葵的HPLC指纹图谱。讨论采用HPLC构建"紫背天葵"指纹图谱,探讨指纹图谱的技术要点及检测条件,以能反映该品种整体特征的HPLC指纹图谱作为质量评价方法,对现有质量控制方法作出积极而又必要的补充和提高,为建立统一的质量标准奠定基础,从而建立新的质量标准。Objective To study high performance liquid chromatography of Begonia fimbristipula（HPLC） fingerprint. Methods Uses the chromatographic column Shim-pack VP-ODS（250 mm×4.6 mm,5 μm）;H3p O4 at 0.5%（V/V）as mobile phase A,with 50% acetonitrile as mobile phase B（V/V）,with gradient elution;The flow rate of 0.8 ml/min;column temperature 30 ℃;detection wavelength of 280 nm;ample quantity 10 μl;the running time of 80 min.Theoretical plate number according to the knapweed-3-O-glycosidase calculation,is not less than 3000.Result The HPLC fingerprint of Begonia fimbristipulata Hance is established.Conclusion The significance of establish the Begonia fimbristipulata Hance HPLC fingerprint：use the High performance liquid chromatography（HPLC）to construct ＂Begonia fimbristipulata Hance＂ fingerprint,discusses the technical key points of fingerprints and testing conditions,to reflect the overall characteristics of the HPLC fingerprint of breed as quality evaluation method,respond positively to the existing quality control method and the necessary supplement and improve,in order to establish the quality standard of unified foundation,so as to set a new standard of quality.

关 键 词：紫背天葵 高效液相色谱法 指纹图谱 

分 类 号：R927.11[医药卫生—药学]