7种牛奶蛋白基因在大肠杆菌中的异源表达  

Synthesis of Seven Milk Proteins in Escherichia Coli

作　　者：张齐[1] 崔金明[1] 蒙海林[1] 张炳照[2] 魏婷[2] 黄建东[2,3] 刘陈立[1,2] ZHANG Qi CUI Jinming MENG Hailin ZHANG Bingzhao WEI Ting HUANG Jiandong LIU Chenli(Bioengineering Research Center, Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences Guangzhou 511458, China Center of Synthetic Biology Engineering Research, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences, Shenzhen 518055, China HKULi Ka Shing Faculty of Medicine, Hong Kong 999077, China)

机构地区：[1]广州中国科学院先进技术研究所生物工程研究中心,广州511458 [2]中国科学院深圳先进技术研究院合成生物学工程中心,深圳518055 [3]香港大学李嘉诚医学院,香港999077

出　　处：《集成技术》2016年第6期79-84,共6页Journal of Integration Technology

基　　金：广州市科技计划项目(201508020092);深圳市未来产业发展专项资金(海洋类)第三批扶持计划(CXZZ20140901004122088);广东省科技计划项目(2014A020216029;2014B020201001;2014A030304008;2014B050505003);深圳科创委基础研究(JCYJ20150521144320992);深圳市科技创新委员会孔雀团队(KQTD2015033117210153)

摘　　要：通过将牛奶中的αs1-酪蛋白、αs2-酪蛋白、β-酪蛋白、κ-酪蛋白、α-乳清蛋白、β-乳球蛋白及白蛋白共7种主要蛋白基因在大肠杆菌中进行了异源表达,并对其中的三种乳清蛋白进行了Western-blot验证。研究结果显示,大肠杆菌可成功表达7种牛奶蛋白,且未被降解。这表明将大肠杆菌作为产生重组牛奶蛋白的底盘细胞具有较好的应用潜能,以上研究对后续进一步开发人造牛奶奠定了基础。Seven main cow milk protein genes, including αs1CN, αs2CN, βCN, κCN, αLa, βLg, and BSA（Bovine Serum Albumin）, were expressed in Escherichia coli and three whey proteins were examined by western-blot. As a trial substage, these genes of seven cow milk protiens expressed successfully in E. coli wihout a phenomenon of degradation. The results indicated that it is initial feasible to gain recombinant milk proteins using recombinant E. coli, and this work lays foundations for the production of cow free milk.

关 键 词：大肠杆菌 WESTERN-BLOT 重组牛奶蛋白 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] R378.2[轻工技术与工程—食品科学与工程]