二氢杨梅素对小鼠局灶性脑缺血再灌注损伤炎症反应的影响  

Dihydromyricetin Reduces Inflammatory Reaction Induced by Focal Cerebral Ischemia-Reperfusion Injury in Mice

作　　者：邓之婧 陈家欢[1] 黄志明[1] 黄晓亮[1] 庞逸敏[1] 郭哲[2] 何萍[3] 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西医科大学第三附属医院,广西南宁530021 [3]广西医科大学实验动物中心,广西南宁530021

出　　处：《中风与神经疾病杂志》2016年第11期973-975,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金(No.81360056);广西自然科学基金面上项目(No.2013GXNSFAA019147);广西自然科学基金回国基金项目(No.2012GXNSFCA053005);南宁市科学研究与技术开发计划(No.20153105)

摘　　要：目的观察二氢杨梅素(dihydromyricetin,DMY)对小鼠局灶性脑缺血再灌注(ischemia/reperfusion,I/R)损伤后炎症反应的影响。方法雄性昆明种小鼠随机分为3组,即假手术组、I/R模型组及DMY(500 mg/kg)组。改良线栓法制备小鼠大脑中动脉阻塞/再灌注(middle cerebral artery occlusion/reperfusion,MCAO/R)局灶性脑缺血(3 h)再灌注(24 h)损伤模型。术前10 d开始灌胃给药,每天1次,并在缺血前1 h及再灌注后12 h各给药1次。再灌注24 h后取脑,免疫组织化学检测小胶质细胞标记物离子钙接头蛋白(ionized calcium binding adaptor molecule-1,Iba1)和5-脂氧合酶(5-lipoxygenase,5-LOX)表达;ELISA法检测缺血脑组织匀浆炎症因子TNF-α和5-LOX代谢物白三烯B4(leukotrienes B4,LTB4)、半胱氨酰白三烯(cysteinyl leukotrienes,CysLTs)含量。结果与模型组比较,DMY 500 mg/kg抑制小胶质细胞活化并降低TNF-α水平,降低缺血脑组织5-LOX蛋白表达并减少LTB4与CysLTs的产生(P<0.05或P<0.01)。结论 DMY可减轻局灶性脑I/R损伤炎症反应,与抑制5-LOX有关。Objective To study the influences of dihydromyricetin （ DMY） on inflammatory reaction induced by fo-cal cerebral ischemia-reperfusion （ I/R） injury in mice.Methods Male Kunming mice were randomly divided into 3 groups：sham group,I/R group and DMY 500 mg/kg group.Mice model of middle cerebral artery occlusion （MCAO,3 h）/reperfusion （24 h） model was established by the improved intraluminal filament technique .Drugs were given orally 10 d （once a day ） before the surgery ,1 h before ischemia and 12 h after reperfusion .After reperfusion for 24 h,the expressions of ionized calcium binding adaptor molecule-1（ Iba1） and 5-LOX in mice brain were assessed by immunohistochemistry;the contents of TNF-αand 5-LOX metabolites （ LTB4 and CysLTs ） in ischemic brain tissue were detected by ELISA .Results Compared with the I/R group,DMY at 500 mg/kg could obviously inhibit the activation of microglia ,lower the level of TNF-α,and reduce the expression of 5-LOX and the production of LTB4 and CysLTs （P〈0.05或P〈0.01）.Con clusion DMY reduces inflammatory reaction induced by focal cerebral I /R injury,which might be associated with its inhibition on 5-LOX.

关 键 词：二氢杨梅素 脑缺血再灌注损伤 炎症反应 5-脂氧合酶 

分 类 号：R966[医药卫生—药理学] R743[医药卫生—药学]