骨诱导性磷酸钙陶瓷材料修复牙槽突裂  

Osteoinductive calcium phosphate ceramics repair alveolar cleft defects

作　　者：陈浩东[1] 姚金凤[2] 梁志刚[2] 

机构地区：[1]广州医科大学,广东省广州市510000 [2]深圳市第二人民医院,广东省深圳市518000

出　　处：《中国组织工程研究》2016年第47期7034-7042,共9页Chinese Journal of Tissue Engineering Research

基　　金：深圳市科技创新委员会基础研究计划(JCYJ20130401111426275;JCYJ20140414170821224)~~

摘　　要：背景:前期研究制备出异位成骨佳的骨诱导性磷酸钙陶瓷。目的:观察骨诱导性磷酸钙陶瓷材料修复牙槽突裂的效果。方法:取比格幼犬9只,建立双侧牙槽突裂模型,造模后3个月,随机选取一侧置入骨诱导性高分子表面双相磷酸钙陶瓷(实验组),另一侧置入非骨诱导性光滑表面双相磷酸钙陶瓷(对照组),同时在犬大腿肌袋内埋置对应的材料。置入后4,8,12周,荧光显微镜下观察牙槽突裂植骨区材料内新骨形成,光镜下观察牙槽突裂植骨区与肌肉内成骨情况,CT检测牙槽突裂恢复情况。结果与结论:(1)荧光显微镜观察结果:两组牙槽突裂植骨区材料内均可同时观察到呈环状排列的红色、黄色和绿色荧光条带;(2)光镜观察结果:置入12周时,实验组牙槽突裂植骨区骨改建明显,材料逐渐分解,间隙由大量成熟骨组织充填,与剩余材料结合紧密,并可见大量典型哈弗氏管结构;在肌肉组织中可见异位成骨。置入12周时,对照组骨组织形态与实验组相近,但成骨速度及成骨量较少,并且肌肉内无异位成骨;(3)CT检测结果:两组材料均很好恢复了犬牙槽突裂的外形和连续性,且不影响缺隙两侧恒牙的正常萌出;(4)结果表明:与传统磷酸钙陶瓷材料相比,骨诱导性磷酸钙陶瓷材料修复牙槽突裂缺损成骨启动更早、成骨速度更快、成骨量更多。BACKGROUND：In our previous studies, we had prepared calcium phosphate ceramics with better ectopic osteogenesis. OBJECTIVE：To explore the effect of novel osteoinductive calcium phosphate ceramics in the repairing of alveolar cleft. METHODS：Bilaterl alveolar defects were created in nine immature beagles. Three months later, osteoinductive calcium phosphate ceramics with high modular surface （experimental group） and smooth surface （control group） were randomly implanted in each side of the defect. Meanwhile, the corresponding material was implanted into the thigh muscle. New bone formation in the implanted region, osteogenesis in the implanted region and muscle, and respair results were respectively observed by fluorescence microscope, light microscope and CT at 4, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION：（1） Fluorescence microscope observation：A circular permutation of red, yel ow and green fluorescent strip could be observed in both two groups. （2） Light microscope observation：At 12 weeks after implantation, in the experimental group, the bone reconstruction was obvious, the implant material was decomposed gradual y, the gap was fil ed with a large number of mature bone that combined with the rest material closely, and numerous Haversian canals appeared;the control group was similar but slightly inferior to the experimental group in the quality of new bone. The experimental group material successful y induced heterotopic osteogenesis in muscle, while the control did not. （3） CT examination：The two group materials restored the appearance and continuity of the alveolar ridge, and made no effect on the eruption of permanent teeth in both sides of the defect. To conclude, our findings suggest that the novel osteoinductive calcium phosphate ceramic exhibits advantages in alveolar cleft repair with earlier osteogenesis activation, faster osteogenesis rate and more bone formation than those traditional materials.

关 键 词：生物材料 口腔生物材料 磷酸钙陶瓷 牙槽突裂 骨诱导 人工骨 生物矿化 

分 类 号：R318[医药卫生—生物医学工程]