羟基乙酸负载软骨细胞组织工程软骨修复喉软骨缺损  

Glycolic acid loaded with chondrocytes repairs laryngeal cartilage defects

作　　者：贾敏[1] 袁琨[1] 

机构地区：[1]华中科技大学同济医学院附属武汉市中心医院,湖北省武汉市430014

出　　处：《中国组织工程研究》2016年第47期7051-7056,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:三维可降解生物支架材料的选择对组织工程软骨的构建十分重要,它能为细胞生长提供依附的支架及增殖空间。目的:观察羟基乙酸负载软骨细胞修复喉软骨缺损的效果。方法:取新西兰白兔60只,随机分为2组,对照组、实验组建立喉软骨缺损模型,实验组于缺损处植入负载软骨细胞的羟基乙酸,对照组缺损处植入羟基乙酸,植入后4,8周进行大体与组织学观察。结果与结论:(1)大体观察结果:植入后4周,对照组缺损部位色泽暗红,创面明显,与周围组织界限清晰;实验组缺损部位色泽暗红,表面光滑,与周围软骨平齐;(2)甲苯胺蓝染色结果:植入后8周,实验组喉软组织缺损部位未见明显炎症反应,缺损部位无明显软骨陷窝,有大量软骨细胞生成;对照组缺损部位存在轻微炎症反应,缺损部位存在轻微陷窝,有少许软骨细胞生成;(3)糖胺聚糖及Ⅱ型胶原蛋白阳性面积:实验组植入4,8周的糖胺聚糖及Ⅱ型胶原蛋白阳性面积大于对照组(P<0.05);(4)结果表明:羟基乙酸负载软骨细胞的组织工程软骨可促进喉软骨缺损的修复。BACKGROUND：A three-dimensional biodegradable scaffold is important for tissue-engineered cartilage construction, and it that can provide conditions for cel attachment and proliferation. OBJECTIVE：To observe the treatment outcomes of glycolic acid loaded with chondrocytes in laryngeal cartilage repair. METHODS：Sixty New Zealand white rabbits were enrol ed and randomly divided into control and 〈br〉 experimental groups. Laryngeal cartilage defect models were established in each group, fol owed by implanted with glycolic acid loaded with chondrocytes and glycolic acid, respectively. Gross and histological observations were conducted at 4 and 8 weeks after implantation. RESULTS AND CONCLUSION：Gross observation showed that at 4 weeks after implantation, a deep red wound with an obvious boundary was seen in the control group;the dark red and smooth defect paral el to the surrounding tissue was found in the experimental group. Toluidine blue staining revealed that at 8 weeks after implantation, the laryngeal defect site showed no obvious inflammation and cartilage col apse, with numerous newly-formed chondrocytes in the experimental group;in contrast, mild inflammation and cartilage col apse were found in the defect region of the control group, and few newly-formed chondrocytes appeared. The positive areas of glycosaminoglycan and type II col agen in the experimental group were significantly larger than those in the control group at 4 and 8 weeks after implantation （P〈0.05）. These results indicate that glycolic acid loaded with chondrocytes contributes to the repair of laryngeal cartilage defects.

关 键 词：生物材料 软骨生物材料 羟基乙酸 软骨细胞 喉软骨缺损 喉软骨缺损模型 胶原蛋白 甲苯胺蓝染色 

分 类 号：R318[医药卫生—生物医学工程]