医院环境分离铜绿假单胞菌的耐药性观察  

Observation on antibiotic resistance of Pseudomonas aeruginosa isolated from hospital environments

作　　者：王会平[1] 张洪江[1] 董歧[1] 刘洁[1] 葛军旗[1] 段杉[1] 王仲华[1] 张政[1] 

机构地区：[1]北京市朝阳区疾病预防控制中心,北京100021

出　　处：《中国消毒学杂志》2016年第11期1072-1074,1078,共4页Chinese Journal of Disinfection

摘　　要：目的研究医院物体表面和医护人员手上携带铜绿假单胞菌的分布及其耐药性,为有效控制医院感染提供参考。方法通过病原学标本监测和药敏试验方法,对北京市朝阳区医疗机构物体表面和医务人员手标本进行检测与分析。结果在床单位、抹布、暖水瓶和医务人员手等4种表面中,以床单位表面铜绿假单胞菌分离率最高,普通病房物体表面携带铜绿假单胞菌较重症监护病房者严重。从医院环境中分离的24株铜绿假单胞菌对氨苄西林等9种抗菌药物全部耐药。24株铜绿假单胞菌全部携带mir基因,sul1和tem-1基因携带率超过70%。结论铜绿假单胞菌在医院环境表面分布较广,呈现出多重耐药现象,应加强医院环境卫生学管理以有效降低环境污染菌数量。Objective To investigate the distribution and antibiotic resistance of Pseudomonas aeruginosa （PAE） isolated from object surfaces and medical staff＇s hands in hospitals, and to provide reference for effective control of hospital infection. Methods By means of pathogenic monitoring and drug sensitivity test,the specimens of the medical institutions in Chaoyang District Beijing,were detected and analyzed. Results The proportion of PAE in bed unit was significantly higher than that in other sources, such as medical staff＇s hands, dusters and thermos bottles. The detection rate of PAE in general wards was significantly higher than that in intensive care units. 24 strains of PAE were all resistant to 9 kinds of antibiotics such as Ampicillin. mir gene was detected in all 24 strains of PAE. The positive rates of sulland temlgenes were both over 70%. Conclusion PAE distributes widely on the surface of hospital environments and appears multidrug resistance. It is necessary to strengthen environmental hygiene management of the hospital to reduce the environmental pollution bacteria.

关 键 词：医院环境表面 铜绿假单胞菌 耐药性 耐药基因 监测 

分 类 号：R378[医药卫生—病原生物学]