运动训练和表没食子儿茶素没食子酸酯对2型糖尿病大鼠肾脏TLR4炎性因子表达的作用研究  

Effect of Exercise Training and Egcg in Type 2 Diabetic Rat Kidney Tlr4 Expression of Inflammatory Cytokines

作　　者：习雪峰[1] 侯改霞[1] 刘倩倩[1] 张林[2] 

机构地区：[1]河南大学体育学院,河南开封475001 [2]苏州大学体育学院,江苏苏州215021

出　　处：《西安体育学院学报》2016年第6期726-731,共6页Journal of Xi'an Physical Education University

基　　金：河南省教育厅科学技术研究重点项目(14B890010;14B890009)

摘　　要：目的探讨运动训练、表没食子儿茶素没食子酸酯(EGCG)单独干预及联合应用对2型糖尿病大鼠肾脏血管通透性和肾TLR4、NF-κBp65、IL-1β蛋白表达量的影响。方法通过高脂喂养加腹腔注射小剂量链脲佐菌素(STZ)构建2型糖尿病SD大鼠模型。将造模成功的56只大鼠随机分为糖尿病对照组(D)、糖尿病给药组(DY)、糖尿病运动组(DE)、糖尿病运动给药组(DEY),每组14只,另设正常对照组(C)12只。运动组大鼠进行12周不负重游泳训练,每周训练5 d。EGCG给药组大鼠每周灌胃7 d。实验结束后检测大鼠FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性变化,并对实验结果进行统计分析。结果与正常对照组(C)相比,糖尿病对照组(D)的FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性均显著升高(P<0.01);糖尿病给药组(DY)、糖尿病运动组(DE)、糖尿病运动给药组(DEY)的FBG、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白和肾脏血管通透性较糖尿病对照组(D)均显著降低(P<0.05或P<0.01);糖尿病运动给药组(DEY)FBG、UAER、TLR4、NF-κBp65、IL-1β蛋白表达量和肾血管通透性均显著低于糖尿病给药组(DY)(P<0.05或P<0.01),糖尿病运动给药组(DEY)肾脏指数、尿蛋白、UAER、TLR4、NF-κBp65、IL-1β蛋白表达量和肾血管通透性均显著低于糖尿病运动组(DE)(P<0.05)。结论 2型糖尿病大鼠存在肾组织炎性因子表达增多,运动训练和EGCG可改善2型糖尿病大鼠肾脏炎性损伤,抑制TLR4信号通路。In order to investigate the impact of exercise and EGCG on vascular permeabilityTLR4 、 NF-κBp65 and IL-1β expression of diabetic nephropathy. Methods ： They were randomly divided into group D, DY, DE, DEY, and additionally provid- ed a c, omparison group C. The rats of group DE were arranged to do non-intensive swimming exercise 5 days for 12 weeks while and the rats of group were given gastry lavage for 7 days per week. After the experiment, rats＇ FBG 、 Urinary protein , UAER,TLR4,NF-κBp65 ,IL-1β were tested. Results： Compared with the control group （C）, diabetic control group （D）of the FBG, urine protein, UAER, TLR4, NF-κBp65, IL-1 β protein and renal vascular permeability were significantly higher （P 〈0 01 ） ; diabetes treatment group （DY）, diabetes exercise group （DE）, diabetes sports administration group （DEY）of FBG, urine protein, UAER, TLR4, NF-κBp65, IL-1β protein and renal vascular permeability resistance than diabetic control group （D）were significantly lower （P 〈 0. 05 or P 〈 0. 01 ） ; diabetes treatment group movement （DEY）FBG, UAER, TLR4, NF-κBp65, IL-1β protein expression and renal vascular permeability were significantly lower in diabetic treatment group （DY） （P 〈 0. 05 or P 〈 0. 01 ）, diabetes, sports administration group （DEY） index kidneys, urine protein, UAER, TLR4, NF-κBp65, IL-1β protein and renal expression of vascular permeability diabetes were significantly lower than the exercise group （DE）（P 〈 0.05 ）. Conclusion： Type 2 diabetic rat kidney tissue increased expression of inflammatory cyto- kines, exercise and EGCG can improve the inflammatory type 2 diabetic rats, inhibit TLR4 signaling pathway.

关 键 词：2型糖尿病 运动训练 EGCG 炎性 血管通透性 

分 类 号：G804.7[文化科学—运动人体科学]