土炒白术HPLC指纹图谱研究  

HPLC Fingerprint for Soil-processed Atractylodis Macrocephalae Rhizoma

作　　者：李慧峰[1] 荆然[1] 程艳刚 裴妙荣[1] 

机构地区：[1]山西中医学院,山西晋中030619 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《山东中医药大学学报》2016年第6期570-572,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山西省中药材;中药饮片地方标准研究项目(编号:2013003B)

摘　　要：目的 ：建立土炒白术的HPLC指纹图谱,为全面控制土炒白术的质量提供方法。方法 ：采用Brava BDS C18（250 mm×4.6 mm,5μm）色谱柱;甲醇（A）-水（B）梯度洗脱;流速：1.0 m L·min-1;检测波长：220 nm,建立土炒白术的HPLC指纹图谱。结果 ：标定了土炒白术中的14个共有峰,非共有峰面积小于10%;10批药材成分的相似度较高,RSD为0.902%-0.997%。结论：该方法简便,重复性好,特征性强,可用于土炒白术药材的鉴别和质量控制。Objective：To establish the fingerprints and provide the method for the quality control of soil-processed Atractylodis macrocephalae Rhizoma by HPLC. Methods：The separation was performed on Brava BDS C18 column（250mm×4.6 mm,5 μm）,with methanol（A）-water（B） as mobile phase by gradient elution,the flow rate was 1.0mL·min-1,the detection wavelength was 220 nm. Results：Total 14 common peaks were selected in Soil-processed Atractylodis macrocephalae Rhizoma,the non-common peak area was less than 10%,the ten batches of herbs were with very high similarity,RSD was 0.902% - 0.997% . Conclusion：The method was convenient and with high repeatability and characteristic,could be used for the identification and quality control of soil-processed A tractylodis macrocephalae Rhizoma.

关 键 词：土炒白术 HPLC 指纹图谱 5-羟甲基糠醛 质量标准 

分 类 号：R286.0[医药卫生—中药学]