通过流式分选技术筛选HIV-1特异性单个B细胞  

Separation and screening of HIV-1 specific single B cell through flow cytometry

作　　者：李丹[1] 鞠斌[1] 王硕[1] 李亚锋[1] 梁华[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,传染病预防控制国家重点实验室,感染性疾病诊治协同创新中心,北京102206

出　　处：《中国热带医学》2016年第11期1074-1076,共3页China Tropical Medicine

基　　金：国家自然基金(No.31600734);传染病预防控制国家重点实验室基金(No.2012SKLID103,2015SKLID506);国家“十二五”重大传染病防治研究项目(No.2014ZX10001001-002)

摘　　要：目的探讨流式细胞分选技术在筛选HIV-1抗原特异性单个B细胞中的应用。方法通过HIV-1特异性探针结合多色流式细胞技术,标记HIV-1抗原特异性B细胞;并结合单细胞流式分选,从两例HIV-1慢性感染者体内分离抗原特异性单个B细胞。结果两个样本均有较强的中和活性,可以用作筛选中和抗体。两例感染者经流式分选后分别获得探针特异性目的细胞CD3-、CD8-、INDO-1 VIOLET-、CD14-、CD19+、CD20+、Ig M-、Ig G+、JRFLgp140+、JRFLgp140-D368R-各33和20个。结论通过流式分选技术结合HIV-1特异性探针可以获得单个HIV-1抗原特异性B细胞,为后续获得有广谱中和HIV-1能力的抗体,设计抗HIV-1药物和HIV-1疫苗免疫原提供技术支持。Objective We explored the application of flow cell sorting in screening HIV- 1 specific single B cell.Methods HIV-1 antigen specific single B cell was separated through multicolor flow, single B cell sorting combined withHIV-1 specific probe screening from two chronic HIV-1 infected patients. Results Two samples had a strong neutralizedactivity, and could be used as a screening of neutralizing antibodies. Totally 33 and 20 HIV-1 specific B cells（CD3^-, CD8^-,INDO-1 VIOLET^-, CD14^-, CD19^＋, CD20^＋, Ig M^-, Ig G^＋, JRFLgp140^＋, JRFLgp140^-D368R^-） were got respectively from thepatients. Conclusion The results demonstrate that single HIV-1 specific B cell could be got through the cell sorting, whichcould acquire the broad neutralizing antibody and provide solid basis for antibody-related drugs and novel immunogens.

关 键 词：流式分选 HIV-1 探针 单个B细胞 

分 类 号：R593.3[医药卫生—内科学]