牛干扰素诱导跨膜蛋白3对口蹄疫病毒感染的抑制作用  

Bovine interferon-induced transmembrane protein 3 inhibits foot-and-mouth disease virus infection

作　　者：赵飞[1] 杜寿文[1] 谭鹏[1] 曹婷婷[1] 许汪[1] 邢彬[1] 王茂鹏[1] 朱羿龙[1] 刘立明[2] 赵翠青[2] 李昌[1,2,3] 金宁一[1,2,3] ZHAO Fei DU Shou-wen TAN Peng CAO Ting-ting XU Wang XING Bing WANG Mao-peng ZHU Yi-long LIU Li-ming ZHAO Cui-qing LI Chang JIN Ning-yi(Key Laboratory of Zoonosis Prevention and Control of Jilin Province ,Military Veterinary Institute of the Academy of Military Medical Sciences, Changchun 130122, China Institute of Virology, Wenzhou University ,Wenzhou , Zhejiang 225035, China Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses , Yangzhou , J iangsu 225009 ,China)

机构地区：[1]军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室,吉林长春130122 [2]温州大学病毒学研究所,浙江温州325035 [3]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医学报》2016年第12期2035-2041,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31472197);病原微生物生物安全国家重点实验室开放课题资助项目(SKLPBS1435);北京市自然科学基金资助项目(5152023)

摘　　要：为了研究牛干扰素诱导跨膜蛋白3(bIFITM3)对O型口蹄疫病毒(FMDV)的抑制作用,本试验将表达bIFITM3的重组质粒pLV-bIFITM3和表达增强型绿色荧光蛋白(EGFP)的对照质粒pLV-EGFP分别转染BHK-21细胞,通过嘌呤霉素抗性筛选获得能够稳定表达外源基因的细胞系。用O型FMDV感染稳定细胞系,通过观察细胞病变、噬斑分析和实时荧光定量PCR方法评价bIFITM3对O型FMDV感染的抑制作用。结果表明,成功筛选获得稳定表达bIFITM3与EGFP的BHK-21细胞系,bIFITM3表达后显著抑制FMDV感染BHK-21细胞,且抑制作用在病毒感染循环的早期阶段就已显现。本试验证明bIFITM3在FMDV感染中具有抗病毒作用,为口蹄疫的防控研究提供新的思路和理论依据。To study the inhibitory effect of Bos taurus interferon-inducible transmembrane protein 3 （bIFITM3） on foot-and-mouth disease virus （FMDV） type O. The recombinant plasrnids of pLV- bIFITM3 expressing bIFITM3 and pLV-EGFP expressing EGFP were separately transfected into BHK-21 cells. BHK-21 cell stains with stable expression of exogenous gene were screened by puro- mycin resistance. The cell stains were infected by FMDV type O by means of observation of cyto- pathic effect under microscope, plaque assay and RT-qPCR to assess the impact of bIFITM3 on FMDV infection. The results showed that BHK-21 cell lines stably expressing bIFITM3 and EGFP were successfully screened,the expression of bIFITM3 displayed strong restriction effect on FM- DV infection which appeared in the early stages of FMDV infection cycle. This was the first report on that bIFITM3 have antiviral activity in FMDV infection, providing new ideas and theoretical basis for the research on the prevention and control of FMD.

关 键 词：牛干扰素诱导跨膜蛋白3 口蹄疫病毒 BHK-21细胞 

分 类 号：S852.65[农业科学—基础兽医学] S852.4[农业科学—兽医学]