丙泊酚对鼠胚胎神经干细胞凋亡的影响及作用机制  

Effects of Propofol on embryonic neural stem cells apoptosis and its mechanism in rats

作　　者：肖华平[1] 程其许[1] 杨小敏[1] 

机构地区：[1]江西省肿瘤医院麻醉科,江西南昌330029

出　　处：《广州医科大学学报》2016年第4期9-13,共5页Academic Journal of Guangzhou Medical University

基　　金：江西省自然科学基金(20122BAB215036)

摘　　要：目的：探讨丙泊酚对鼠胚胎神经干细胞凋亡的影响及作用机制。方法：选择孕14～16 d Wistar大鼠，分离培养胚胎神经干细胞，分别用5、25、50、100μmol／L丙泊酚进行处理，同时设立脂肪乳剂组和空白对照组。采用MTT法检测细胞存活率；流式细胞术观察丙泊酚对胚胎神经干细胞凋亡的影响；提取对照组、脂肪乳剂组、50μmol／L丙泊酚、50μmol／L丙泊酚＋caspase抑制剂培育12 h后的全细胞蛋白， Western-blot检测激活的细胞色素c、caspase-3、caspase-8、caspase-9蛋白的表达。结果：丙泊酚可引起胚胎神经干细胞凋亡，其效应呈剂量依赖型（P＜0．05）；丙泊酚处理后的胚胎神经干细胞激活的 caspase-3、CytochromeC、caspase-9、caspase-8蛋白表达水平显著增加（P＜0．05）。结论：丙泊酚主要通过激活caspase依赖的内源性和外源性凋亡通路引起鼠胚胎神经干细胞凋亡。Objective：To investigate the effects of Propofol on embryonic neural stem cells apoptosis and its mechanism in rats.Methods：The pregnant 14-16 d Wistar rats were included. The embryonic neural stem cells were isolated and cultured,and treated with 5,25,50 and 100 μmol/L Propofol,respectively. The fat emulsion group and the control group were established. The cell viability was measured by MTT assay. The effect of Propofol on embryonic neural stem cells apoptosis was determined by flow cytometry. After cultured for 12 h,the whole-cell proteins in the control group,fat emulsion group,50 μmol/L Propofol group,50 μmol/L Propofol＋caspase inhibitor group were extracted. The protein expressions of the activated cytochrome c,caspase-3,caspase-8 and caspase-9 were determined by Western blot. Results：Propofol can cause embryonic neural stem cell apoptosis,and its effect was dose-dependent （P〈0.05）. The expression levels of the activated embryonic neural stem cells caspase-3,Cytochrome C,caspase-9 and caspase-8 protein were significantly increased after Propofol treatment （P〈0. 05）. Conclusion：Propofol causes embryonic neural stem cells apoptosis mainly through the activation of caspase-dependent intrinsic and extrinsic apoptotic pathways.

关 键 词：丙泊酚 胚胎神经干细胞 流式细胞术 凋亡 

分 类 号：R614[医药卫生—麻醉学]