长期尼古丁摄入对大鼠骨内微结构及机体氧化应激状态影响的实验研究  被引量:4

EXPERIMENTAL STUDY ON EFFECT OF NICOTINE INTAKE ON IMPACT OF BONE MICROSTRUCTURE AND OXIDATIVE STRESS IN RATS

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作  者:杨阳[1] 王亚薇[2] 马信龙[1] 马剑雄[3] 邢丹[4] 朱少文[1] 陈阳[1] 

机构地区:[1]天津市天津医院骨科,天津300211 [2]天津市天津医院肌电图室,天津300211 [3]天津市中西医结合骨科研究所生物力学实验室 [4]北京大学人民医院关节外科

出  处:《中国修复重建外科杂志》2016年第12期1493-1497,共5页Chinese Journal of Reparative and Reconstructive Surgery

基  金:国家自然科学基金资助项目(81572154;81501919);天津市卫生和计划生育委员会攻关项目(15KG123);天津市卫生和计划生育委员会科技基金项目(2014KY31)~~

摘  要:目的探讨长期尼古丁摄入对大鼠骨内微结构、骨生物力学性能以及氧化应激状态的影响。方法取6周龄雄性sD大鼠36只,体质量160~180g,随机分为3组,分别为对照组、低剂量组和高剂量组,每组12只。高剂量组和低剂量组分别按照每天6.0、0.4mg/kg标准尼古丁灌胃干预,持续12个月;对照组不作处理。实验期间观察各组大鼠存活情况,每个月大鼠称重。造模12个月后处死全部大鼠,取L1椎体进行Micro—CT三维重建测量骨密度(bonemineral density,BMD)、骨体积分数(bone volume fraction,BVF)、骨小梁厚度(trabecular thickness,TT)、骨小梁数目(trabecular number,TN)、骨小梁间隙(trabecular spacing,TS),取左侧股骨干进行生物力学测试最大载荷、抗弯刚度、最大断裂能;取动脉血检测丙二醛(malonyldialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)以及可替宁含量。结果实验期间,高剂量组死亡2只大鼠,低剂量组死亡1只,对照组大鼠均存活。随时间延长,高、低剂量组大鼠体质量逐渐低于对照组,8—12个月时,高、低剂量组与对照组比较差异有统计学意义(P〈0.05);11、12个月时,高剂量组大鼠体质量明显低于低剂量组(P〈0.05)。造模12个月后,高剂量组BMD、BVF、TT、TN均显著低于对照组、低剂量组,Ts显著增高(P〈0.05);低剂量组仅BVF、TN、TS与对照组比较差异有统计学意义(P〈0.05)。高剂量组股骨干最大载荷、抗弯刚度以及最大断裂能明显低于对照组、低剂量组,低剂量组明显低于对照组(P〈0.05)。与对照组比较,高、低剂量组可替宁以及MDA含量均明显升高,CAT、SOD含量明显降低(P〈0.05);且高、低剂量组间差异亦有统计学意义(P〈0.05)。结论长期尼古丁摄入会引起大鼠骨内微结构改变Objective To evaluate the influence of nicotine intake on bone microstructure, bone biomechanics, and oxidative stress state in rats. Methods Thirty-six 6-week-old male Sprague Dawley rats (weight, 160-180 g) were randomly divided into control group, low dose group, and high dose group, 12 rats each group. The rats in high dose group and low dose group were given respectively 6.0 mg/kg and 0.4 mg/kg nicotine gavage intervention for 12 months; no intervention was made in the control group. The survival of rats was observed during experiment, and the weight of rats was measured every month. At 12 months after modeling, the L1 vertebral body was harvested to measure the bone mineral density (BMD), bone volume fraction (BVF), trabecular thickness (TT), trabecular number (TN), and trabecular spacing (TS) by Micro-CT three-dimensional reconstruction; the left femur was harvested for biomechanical tests of maximal load, stiffness, and the maximal fracture energy; and arterial blood was extracted to measure the malonyldialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and cotinine. Results During the experiment,two rats and one rat were added in the high dose group and the low dose group because of death, and no death in the control group. The body weight of the rats in the high and low dose groups gradually decreased with time when compared with one in the control group, and significant difference was found between two dose groups and the control group at 8-12 months (P〈0.05); the body weight of the high dose group was significantly lower than that of the low dose group at 11 and 12 months (P〈0.05). At 12 months after modeling, BMD, BVF, TT, and TN were significantly lower in the high dose group than the control group and the low dose group, but TS was significantly increased (P〈0.05). Difference in BVF, TN, and TS was significant between the low dose group and the control group (P〈0.05). The maximal load, stiffness, and maximal fracture energy of fe

关 键 词:尼古丁 氧化应激 骨质疏松症 生物力学 大鼠 

分 类 号:R580[医药卫生—内分泌]

 

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