Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line  

作　　者：Bratko Filipic Lidija Gradisnik Eva Ruzic-Sabljic Brabara Trtnik Adriana Pereyra Domen Jaklic Rok Kopinc Jana Potokar Almin Puzic Hrvoje Mazija 

机构地区：[1]Institute for Microbiology and Immunology, Medical Faculty Ljubljana, University of Ljubljana, Zalogka 4, Ljubljana 1105, Slovenia [2]Institute for Biomedical Sciences, Medical Faculty Maribor, University of Maribor, Taborska 8, Maribor 2000, Slovenia [3]Research and Control Department, MEDEX d o.o., Linhartova 49a, Ljubljana 1000, Slovenia [4]Veterinary Stations of Mostar, Bigke Polje 106, Mostar 88000, Bosnia and Herzegovina [5]Croatian Institute for Experimental and Translation Oncology, Koledinedka 3, Zagreb 10040, Croatia

出　　处：《Journal of Agricultural Science and Technology(B)》2016年第1期35-47,共13页农业科学与技术（B）

摘　　要：Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly （R J） and honeys. They all have strong antibacterial properties due to the active substances they contain. This study investigated the effect of combination of water soluble propolis （WSP） Greitl20 or fresh royal jelly （F-RJ） （MiZigoj） and Forest honeys as antibacterial against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, methicillin resistant Staphylococcus aureus （MRSA）, Streptococcus pyogenes, Streptococcus agalactiae and Candida albicans. These substances are also cell growth promoters for human macrophage （TLT） cell line. WSP Greitl20, F-RJ （M） and different Forest honeys were prepared in saline as 10% solutions. The antimicrobial activity was expressed as the minimal inhibitory concentration （MIC） in mg/mL. The growth promotion activity was measured at optical density （OD） 595 nm. The combination ofWSP Greitl20 with different Forest honeys is better than F-RJ （M） in same combination with different Forest honeys. The best antibacterial/antifungal activity was found with the combination of 10% WSP Greit 120 in the Forest honey （1：10） from Italy or Spain. When measuring the growth promoting activity of TLT cell line, the best activity was detected at the combination of 10% WSP Greitl20 in the Forest honey from Italy （GI3 = 0.796 ± 0.014 and GI5 = 1.133± 0.022）. Antimicrobial and growth promoting activities are correlated and WSP-dependent.

关 键 词：PROPOLIS royal jelly Forest honey antimicrobial activity human macrophage cell line cell growth promoting activity. 

分 类 号：S896.3[农业科学—特种经济动物饲养] S859.796[农业科学—畜牧兽医]