板蓝根化学成分与抗流感病毒神经氨酸酶活性相关性的探讨  

Correlation between chemical constituents of Isatidis Radix and bioactivity of neuraminidase on anti-influenza virus

作　　者：池絮影 傅咏梅[2] 崔曰新 张蜀[1] 邓红[1] 

机构地区：[1]广东药科大学广东省药物新剂型重点实验室,广东广州510006 [2]国药集团广东环球制药有限公司,广东佛山528200

出　　处：《中草药》2016年第22期3982-3989,共8页Chinese Traditional and Herbal Drugs

摘　　要：目的探讨板蓝根化学成分与体外抗流感病毒神经氨酸酶(NA)活性的相关性。方法采用UPLC法建立不同产地板蓝根提取液的指纹图谱,并选取已知共有峰进行定量测定,采用试剂盒法测定药物抑制流感病毒NA活性,对化学信息和生物效应进行灰色关联分析和单因素相关分析,以探索其相关性。结果不同产地药材、不同提取方法得到的样品的化学成分量与生物效应均有一定的差异。UPLC指纹图谱各共有峰中已知峰(R,S)-告依春和尿苷与抑制流感病毒NA活性相关性较强,其中(R,S)-告依春量与生物效应显著相关。结论基于NA活性检测结果,初步明确了板蓝根化学成分量与生物效应的相关性,为板蓝根抗病毒有效成分的筛选和提取提供数据参考。Objective To explore the relationship between chemical constituents of Isatidis Radix （IR） and the inhibition ofbioactivity of neuraminidase （NA） on influenza virus in vitro. Methods To establish the chemical fingerprints of IR extraction from different producing areas and determine the contents of common peaks by UPLC. To determine the activity of NA on influenza virus tested with IR by Kit. To analyze the correlation of the chemical information and biological effect by mathematical statistics methods such as grey correlation analysis and one-factor analysis. Results The chemical contents and biological effect detection varied markedly in the products from different regions and by extracting methods. The result of comprehensive analysis showed that uridine and （R,S）-goitrin of common components in UPLC-fingerprints related with the inhibition of bioactivity of NA on influenza virus, and the content of （R,S）-goitrin was correlated significantly to the activity of NA. Conclusion Based on the biological assay of NA on influenza virus, the correlation of the content of chemical components and biological effect is verified preliminarily, with a view to provide a more reliable data and reference for the selection and extraction of antiviral active ingredients from IR.

关 键 词：板蓝根 UPLC 指纹图谱 (R S)-告依春 神经氨酸酶 生物活性 灰色关联分析 单因素相关分析 

分 类 号：R284.2[医药卫生—中药学]