新癀片对牛奶致家兔发热模型的解热作用机制研究  

Antipyretic mechanism of Xinhuang Tablet on fever rabbits induced by milk

作　　者：刘静[1] 吕晓静 刘东博[1] 南淑华 关斌 赖志成 申秀萍[1] 

机构地区：[1]天津药物研究院,天津300193 [2]瑞普(天津)生物药业有限公司,天津300300 [3]厦门中药厂有限公司,福建厦门361100

出　　处：《中草药》2016年第22期4035-4038,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的探讨新癀片对牛奶致家兔发热模型的解热作用机制。方法制备牛奶致家兔发热模型,测定给药后1、4 h体温,并取下丘脑测定与体温相关的白细胞介素1α(IL-1α)、IL-β、IL-6、前列腺素E2(PGE2)、肿瘤坏死因子α(TNF-α)、精氨酸加压素(AVP)、环磷酸腺苷(cAMP)、环磷酸鸟苷(c GMP)、Na+,K+-ATP等指标。结果新癀片给药1、4 h后均能显著降低家兔体温,显著降低家兔下丘脑IL-1α、IL-1β、IL-6、PGE2、TNF-α、cAMP水平及cAMP/c GMP值,降低Na+,K+-ATP活力;给药后4 h能显著升高AVP水平。与吲哚美辛比较,新癀片给药后4 h能进一步降低家兔下丘脑IL-1β、IL-6、cAMP水平及cAMP/c GMP值,差异显著(P<0.05),进一步降低PGE2水平,给药后1 h能显著降低IL-1α水平。结论新癀片的解热作用机制与调节下丘脑中体温调控相关因子IL-1α、IL-1β、IL-6、PGE2、TNF-α、cAMP、cAMP/c GMP、Na+,K+-ATP、AVP有关;其中药成分与吲哚美辛发挥协同作用,从而延长吲哚美辛解热作用时间并且增强吲哚美辛的解热效果。Objective To investigate the mechanism of Xinhuang Tablet on fever rabbits induced by milk. Methods Using the milk-reduced rabbit fever model to research the antipyretic mechanism of Xinhuang Tablet, blood plasma and hypothalamus were collected to measure the indicators related to body temperature, such as IL- 1α, IL- 1β, IL-6, TNF-α, PGE2, cAMP, cGMP, AVP, and Na＋, K^＋-ATP ase. Results In the model of milk-reduced rabbit fever, Xinhuang Tablet can significantly decline the contents of IL-lα, IL-1β, IL-6, PGE2, and TNF-ct in the hypothalamus, decline the content of cAMP and ratio of cAMP/cGMP, decline the energy of Na-K-ATPase, and increase the variety of body temperature regulation related factors, such as content of AVP, in order to play a role of antipyretic. Conclusion The antipyretic mechanism and adjustment of Xinhuang Tablet were associated with body temperature regulation related factors in the hypothalamus, such as IL-1α, IL-1β, IL-6, PGE2, TNF-α, cAMP, cAMP/cGMP, Na^＋, K^＋-ATP ase, and AVP; Its indomethacin can decline the contents of IL-lα, IL-6, PGE2, and TNF-ct, increase the content of AVP, decline the energy of Na^＋, K^＋-ATP ase which plays the antipyretic effects of non-steroidal anti-inflammatory drugs, and the containing Chinese medicines can further decline the contents of IL-1β, IL-6, PGE2, and cAMP as well as the ratio of cAMP/cGMP in the hypothalamus of rabbits, synergy with indomethacin, in order to extend the indomethacin＇s antipyretic action time and enhance the indomethacin＇s strength.

关 键 词：新癀片 解热机制 细胞因子 协同作用 吲哚美辛 白细胞介素 前列腺素E2 肿瘤坏死因子α 精氨酸加压素 环磷酸腺苷 环磷酸鸟苷 Na^+ K^+-ATP 

分 类 号：R285.5[医药卫生—中药学]