羊脆木根皮中1个新的异苯并呋喃内脂类化合物及其细胞毒活性  

A new isobenzofuranlactone from root barks of Pittosporum kerrii and its cytotoxicity

作　　者：杨艳[1] 邢欢欢[1] 马航赢 周玲[1] 李银科[1] 叶艳青[1] 胡秋芬[1] 周敏[1] 李建钢[1,2] 

机构地区：[1]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650500 [2]云南省曲靖市第一人民医院麻醉科,云南曲靖655000

出　　处：《中草药》2016年第23期4134-4136,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(21362044;21032085);云南省高校科技创新团队资助项目(IRTSTYN 2014-11)

摘　　要：目的 对羊脆木Pittosporum kerrii根皮的化学成分进行研究。方法 运用硅胶、凝胶及RP-HPLC等多种色谱技术进行分离纯化，并根据理化性质和波谱数据鉴定化合物的结构。结果 从羊脆木根皮70%丙酮提取物中分离鉴定了3个异苯并呋喃内酯类化合物，分别为5-羟基-6-异戊烯基-异苯并呋喃-1（3H）-酮（1）、5-甲基-6-异戊烯基-异苯并呋喃-1（3H）-酮（2）、5-甲氧基-6-（3-O-乙酰基）-丙酰基-异苯并呋喃-1（3H）-酮（3）。其中化合物1为新化合物，命名为羊脆木素A。并对化合物1进行了细胞毒活性测试，其对NB4、SH-SY5Y、PC3、A549和MCF-7细胞的IC50值分别为3.6、5.2、8.8、5.7和6.0 μmol/L。结论 化合物1~3均为首次从羊脆木中分离得到，化合物1为新化合物，且表现出细胞毒活性。Objective To research the chemical constituents in the root barks of Pittosporum kerrii.Methods The chemical constituents in the root barks of P.kerrii were isolated by silica gel,Sephadex LH-20 column chromatographies,and RP-HPLC methods.Their structures were elucidated using various spectroscopic techniques,including extensive 1D and 2D NMR techniques.Results Three isobenzofuranlactones were isolated from the 70% acetone extract in the root barks of P.kerrii.They were 5-hydroxy-6-prenyl-isobenzofuran-1（3H）-one （1）,5-methyl-6-prenyl-isobenzofuran-1（3H）-one （2）,and 3-（6-methoxy-3-oxo-1,3-dihydroisobenzofuran-5-yl）-3-oxopropyl acetate （3）.Conclusion Compounds 1-3 are isolated from P.kerrii for the first time.Compound 1 is a new compound and it is identified as 5-hydroxy-6-prenyl-isobenzofuran-1（3H）-one,named as kerriin A.And it displays obvious cytotoxicity against NB4,SH-SY5Y,PC3,A549,and MCF7 cells with IC50 values of 1.8,2.6,3.5,4.2,and 2.3 μmol/L,respectively.

关 键 词：羊脆木 异苯并呋喃内脂 细胞毒活性 5-羟基-6-异戊烯基-异苯并呋喃-1(3H)-酮 羊脆木素A 

分 类 号：R284.1[医药卫生—中药学]