复方芪麻胶囊UPLC指纹图谱建立和同时测定8种成分研究  

Fingerprint and eight constituents quantitative determination for Compound Qima Capsule by UPLC

作　　者：孙冬梅[1,2] 林晓燕[3] 江洁怡[1,2] 毕晓黎[1,2] 陈雪[3] 陈秋谷 刘梦云[3,1] 

机构地区：[1]广东省第二中医院(广东省中医药工程技术研究院),广东广州510095 [2]广东省中医药研究开发重点实验室,广东广州510095 [3]广州中医药大学,广东广州510405

出　　处：《中草药》2016年第23期4186-4191,共6页Chinese Traditional and Herbal Drugs

基　　金：广东省中医药局建设中医药强省课题“复方芪麻胶囊”(粤中医办函[2015]102号);广东省科技计划项目(2013B04020040)

摘　　要：目的 建立复方芪麻胶囊（CQC）的UPLC指纹图谱，并同时测定指标成分天麻素、5-羟甲基糠醛（5-HF）、毛蕊异黄酮-7-O-β-D-葡萄糖苷（CG）、野漆树苷、柚皮苷、芒柄花素-7-O-β-D-葡萄糖苷（FG）、毛蕊异黄酮、芒柄花素的量，为评价CQC质量提供依据。方法 分析采用Agilent Eclipse C18柱（100 mm×4.6 mm，3.5 μm），以乙腈-0.05%磷酸水溶液梯度洗脱，体积流量0.3 mL/min，柱温25℃，检测波长为265 nm。结果 得到分离度和重现性良好的CQC UPLC指纹图谱，相似度在0.98以上，标示出22个共有峰；8种成分中天麻素在5.976~29.880 μg/mL、5-HF在10.596~52.980 μg/mL、CG在2.697~13.485 μg/mL、野漆树苷在2.262~11.309 μg/mL、柚皮苷在40.768~203.840 μg/mL、FG在5.825~29.126 μg/mL、毛蕊异黄酮在0.372~1.858 μg/mL、芒柄花素在1.888~9.440 μg/mL线性关系良好，r均〉0.999 9，加样回收率分别为1.04%、1.30%、1.81%、1.41%、1.29%、1.01%、1.48%、1.29%。10批样品中天麻素量在2.883~3.491 mg/g，5-HF量在1.710~3.791 mg/g，CG量在0.107~0.286 mg/g，野漆树苷量在0.157~0.346 mg/g，柚皮苷量在8.853~10.726 mg/g，FG量在0.282~0.692 mg/g，毛蕊异黄酮量在0.097~0.135 mg/g，芒柄花素量在0.041~0.063 mg/g。结论 同时对CQC UPLC指纹图谱和8个指标成分进行分析，方法快速、简便、准确，可以作为有效评价该制剂质量的方法。Objective To establish a UPLC fingerprint of Compound Qima Capsule （CQC）,to determine the eight contents in CQC （gastrodin,5-hydroxymethylfurfura （5-HF）,calycosin-7-O-β-D-glycoside （CG）,rhoifolin,naringin,formononetin-7-O-β-D-glucoside （FG）,calycosin,and formononetin）,and to provide the basis for the evaluation of CQC.Methods The Agilent Eclipse C18（100 mm×4.6 mm,3.5 μm） column was used with a mobile phase of methyl acetonitrile-0.05% phosphoric acid gradient elution,the flow rate was 0.3 mL/min,the column temperature was 25℃,and the detection wavelength was 265 nm.Results The fingerprint chromatography with good resolution and reproducibility included 22 mutual peaks,and the similarity was more than 0.98.Gastrodin,5-HF,CG,rhoifolin,naringin,FG,calycosin,and formononetin were baseline seperated with good linearity relationships between concentration and peak areas over the linear ranges,within 0.976-29.880,10.596-52.980,2.697-13.485,2.262-11.309,40.768-203.840,5.825-29.126,0.372-1.858,and 1.888-9.440 μg/mL （r〉0.999 9）,whose average recoveries were 1.04%,1.30%,1.81%,1.41%,1.29%,1.01%,1.48%,and 1.29%.The contents of 10 batches of gastrodin,5-HF,CG,rhoifolin,naringin,FG,calycosin,and formononetin were 2.883-3.491,1.710-3.791,0.107-0.286,0.157-0.346,8.853-10.726,0.282-0.692,0.097-0.135,and 0.041-0.063 mg/g,respectively.Conclusion The method is rapid,simple,and accurate,and can be used for the quality control of CQC.

关 键 词：复方芪麻胶囊 UPLC 指纹图谱 多成分测定 天麻素 5-羟甲基糠醛 毛蕊异黄酮-7-O-β-D-葡萄糖苷 野漆树 苷 柚皮苷 芒柄花素-7一O一13-D一葡萄糖苷 毛蕊异黄酮 芒柄花素 

分 类 号：R286.02[医药卫生—中药学]