交泰丸对抑郁大鼠行为学及脑内单胺类神经递质的影响  

Effects of Jiaotai Pill on behavior and monoamine neurotransmitters of depression rat model

作　　者：杨帅[1] 潘晔[1] 宋彦奇 高杉[1] 蔡雪朦 高树明[1] 于春泉[1] 

机构地区：[1]天津中医药大学,天津300193

出　　处：《中草药》2016年第23期4218-4223,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81273893)

摘　　要：目的通过研究交泰丸对慢性温和不可预知性应激(CUMS)抑郁模型大鼠行为学及脑内单胺类神经递质水平变化的影响,探讨交泰丸的抗抑郁作用及其机制。方法将开场实验行为比较接近的72只SD大鼠随机分为6组,即对照组、模型组、阳性药组(盐酸氟西汀7.5 mg/kg)和交泰丸低、中、高剂量(生药0.75、1.50、3.00 g/kg)组,每组12只,分笼饲养;除对照组外,其余5组动物给予CUMS刺激制备CUMS抑郁模型,各给药组连续ig给予治疗药物14 d,每组动物每周测定1次体质量及糖水偏好度,于第5周观察大鼠开场实验穿越横格数及直立次数的变化;行为学指标测定完毕后,取脑组织,用HPLC法测定海马组织、皮质组织和下丘脑组织中单胺类神经递质去甲肾上腺素(NE)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)水平的变化。结果与模型组比较,交泰丸低、中、高剂量组大鼠体质量、糖水消耗量以及开场实验中穿越横格数及直立次数均显著增加;且海马组织、皮质组织、下丘脑组织中NE和5-HT水平明显升高,5-HIAA水平均明显降低(P<0.05、0.01)。结论交泰丸具有明显的抗抑郁作用,其机制与上调脑内单胺类神经递质水平有关。Objective To study the effects of Jiaotai Pill on the behavior and monoamine neurotransmitters of chronic mild unpredictable stress （CUMS） depression rat model and to investigate the anti-depression effect and mechanism of Jiaotai Pill.Methods A total of 72 SD rats were divided into six groups randomly,namely control,model,positive drug （fluoxetine hydrochloride,7.5 mg/kg）,low-,mid-,and high-doses of Jiaotai Pill （crude drug 0.75,1.5,and 3 g/kg） groups （n=12）,which were reared in cage and execpt the control group,the other five groups of rats were given CUMS to stimulate the production of CUMS depression model,then ig given Jiaotai Pill for 14 d.Rats in each group were measured the body weight and the rate of sucrose preference once a week to observe the open-field activity behavior （through the horizontal grid and the number of vertical changes） at the 5th week.All the animals were killed to get the blood and brain cortex to test the levels of NE,5-HT,and 5-HIAA in hippocampus,cortex,and hypothalamus by high performance liquid chromatography （HPLC）.Results Compared with the model group,the test indexes of rat body weight,sucrose water consumption,and the open-field activity behavior were significantly increased in the low-,mid-,and high-dose of Jiaotai Pill groups.And the contents of NE and 5-HT increased,while the content of 5-HIAA decreased obviously in the hippocampus,cortex,and hypothalamus in all Jiaotai Pill groups （P〈0.05 or 0.01）.Conclusion Jiaotai Pill has a better antidepressant effect whose mechanism is related to increasing the level of monoamine neurotransmitters in the cerebral cortex.

关 键 词：交泰丸 抑郁症 单胺类神经递质 慢性温和不可预知性应激 去甲肾上腺素 5-羟色胺 5-羟吲哚乙酸 

分 类 号：R285.5[医药卫生—中药学]