应用物种DNA条形码识别太湖流域部分底栖无脊椎动物种类  

Applying DNA Barcoding for Identification of Some Invertebrate Macrozoobenthos at Taihu Lake Basin

作　　者：张翔[1,2,3] 张效伟[1] 杨江华[1] 周国栋 

机构地区：[1]南京大学环境学院,江苏南京210000 [2]江苏省环境保护水环境生物监测重点实验室,江苏常州213001 [3]常州市环境监测中心,江苏常州213001

出　　处：《环境监控与预警》2016年第6期18-21,25,共5页Environmental Monitoring and Forewarning

基　　金：国家水体污染控制与治理科技重大专项基金资助项目(2012ZX07506-003)

摘　　要：将物种DNA条形码实际应用于太湖流域底栖无脊椎动物分类,并与形态学分类结果比对,结果表明,DNA条形码技术可应用于本流域底栖无脊椎动物分类,但现阶段无法替代形态学鉴定,主要原因是太湖流域底栖无脊椎动物的绝大多数物种COⅠ基因特征序列是未知的或是BLAST所拥有的分类程度不够,提出在今后的研究中应探索建立太湖流域底栖无脊椎动物COⅠ基因数据库。DNA barcoding was appl ied for identificat ion of invertebrate macrozoobenthos at Taihu Lake basin and the results were compared with those obtained from morphological classification. The results demonstrated that DNA barcoding could be applied to classify invertebrate macrozoobenthos at the basin, but at this stage could not replace morphological classification. The main reason was that there were unknown COI characteristic gene sequences for most of the invertebrate macrozoobenthos species or that the degree of classification was not enough for BLAST. It was proposed to explore and establish the gene data bank for invertebrate macro-zoobenthos at Taihu Lake basin in future studies.

关 键 词：太湖流域 底栖无脊椎动物 DNA条形码 形态学分类 

分 类 号：X835[环境科学与工程—环境工程]