喀斯特山区2个鲫鱼群体基于转铁蛋白的遗传多样性研究  

Genetic Diversity Study of Two Groups of Carassius auratus in Karst Mountainous Area Based on Transferrin

作　　者：周其椿 安苗[2] 王金娜 郭健康[2] 杨兴 李建光 

机构地区：[1]贵州省水产研究所,贵州贵阳550025 [2]贵州大学动物科学学院,贵州贵阳550025 [3]正安县水产技术推广站,贵州正安563400

出　　处：《河南农业科学》2016年第12期143-148,共6页Journal of Henan Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金项目(CARS-46);贵州省农业科技攻关项目[黔科合NY字(2009)3070];贵州省科学技术基金项目[黔科合J字(2011)2223]

摘　　要：为了解喀斯特山区2个不同鲫鱼群体在蛋白质水平的遗传多样性及其遗传差异,运用聚丙烯酰胺凝胶电泳方法对喀斯特山区2个群体鲫鱼的血清转铁蛋白电泳表型及多样性进行研究。结果表明:群体克隆多样性指数表现为草海鲫鱼(0.32)﹥红鲫(0.25,对照)﹥普安鲫鱼(0.13);3个鲫鱼群体的13个克隆由9个不同的等位基因产生,等位基因的频率分别为Tf^a0.144 9、Tf^b0.202 9、Tf^c0.036 2、Tf^d0.058 0、Tf^e0.007 2、Tf^f0.239 1、Tf^g0.014 5、Tf^h0.275 4、Tf^i0.029 0;普安鲫鱼与草海鲫鱼和红鲫无共享的基因型;普安鲫鱼与草海鲫鱼之间的遗传相似性指数较高(0.714 3),遗传距离较小(0.285 7)。总体来看,草海鲫鱼的克隆多样性水平较高,其转铁蛋白多样性程度较普安鲫鱼丰富;2个群体之间存在较近的亲缘关系,未达到遗传距离种的分化标准。In order to study the genetic diversity and genetic difference at protein levels of two kinds of crucian carp( Carassius auratus) in Karst mountainous area,the electrophoresis phenotype of serum transferrin( Tf) and diversity were studied by the method of polyacrylamide gel electrophoresis. The results showed that the groups clonal diversity index showed Caohai crucian carp( 0. 32) ﹥ Red crucian carp( 0. 25) ﹥ Puan crucian carp( 0. 13). Thirteen phenotypes of three kinds of crucian carp were determined by nine kinds of different alleles,frequencies of allelic genes were Tf^a0. 144 9,Tf^b0. 202 9,Tf^c0. 036 2,Tf^d0. 058 0,Tf^e0. 007 2,Tf^f0. 239 1,Tf^g0. 014 5,Tf^h0. 275 4,Tf^i0. 029 0,respectively.There were none shared genotype among three kinds of crucian carp. They had higher genetic similarity and smaller genetic distances between Puan crucian carp and Caohai crucian carp,which were 0. 714 3and 0. 285 7,respectively. Researches demonstrated that clonal diversity was higher and the transferrin diversity level was richer in Caohai crucian carp than in Puan crucian carp. The genetic diversity was more complicated of Caohai crucian carp,and there were lots of similarities with Puan crucian carp,however,there were also differences between them.

关 键 词：喀斯特山区 鲫鱼 转铁蛋白 遗传多样性 

分 类 号：Q953[生物学—动物学]