matK和rbcL序列在半夏属中的应用  

An Application of matK and rbcL in Pinellia

作　　者：赵月梅[1] 李筱玲[1] 

机构地区：[1]商洛学院生物医药与食品工程学院,陕西商洛726000

出　　处：《商洛学院学报》2016年第6期67-70,共4页Journal of Shangluo University

基　　金：商洛市科学技术研究计划项目(SK2014-01-20)

摘　　要：为了分析半夏属物种之间的亲缘关系,选用了mat K和rbc L序列对半夏属7个种共21个个体进行了分析。经比对后用MEGA5.0计算种间的k2p遗传距离并基于该距离建立了NJ系统发育树。结果表明,只有mat K和rbc L联合序列能分开这7个物种,mat K和rbc L单独序列均不能将这7个物种分开。表明mat K和rbc L联合序列可以作为DNA条形码鉴别半夏属物种。但是对于准确探讨该属的亲缘关系有一定难度。MatK and rbcL gene are used to evaluate the phylogeny of Pinellia （ Araceae） in 21 samples from 7 species. Compared with the divergence of these sequences, the genetic distances were computed using MEGA5.0 in accordance with the Kimura 2 -Parameter （ K2p） model, and the phylogenetic tree was constructed using the neighbor-joining and Kimura 2 -parameter methods. Results showed that only the matK＋rbcL could distinguish 7 species in Pinellia and neither of them could distinguish all the 7 species alone. The matK＋rbcL fragment could be used as a candidate barcode for identifying species of Pinellia, but it is difficult to analyze the phylogeny in Pinellia.

关 键 词：MATK RBCL 半夏属 系统发育 

分 类 号：S567.239[农业科学—中草药栽培]