附子中6种生物碱质谱裂解机制及醇解产物研究  

Study on the Fragmentation Behavior and Alcoholysates of Aconitum Alkaloids in Aconitum Carmichaeli Debx

作　　者：张翅[1,2] 潘德林[1,2] 朱雅宁[1,2] 郝莉 

机构地区：[1]华润三九(雅安)药业有限公司,四川雅安625000 [2]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中国中医急症》2016年第12期2278-2282,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家"重大新药创制"科技重大专项(2013ZX09201018)

摘　　要：目的探讨附子中6种生物碱裂解规律,研究双酯型生物碱乙醇解产物。方法采用HPLC-ESI-MS技术,在正离子模式下,扫描分析双酯型生物碱在乙醇的转化产物,并阐述其规律。结果通过对已知6种生物碱的多级质谱分析及文献调研,总结了6种酯型生物碱的裂解规律,并确定了双酯型生物碱的乙醇产物。结论 3种双酯型生物碱在乙醇中,发生乙醇解反应,分别生成8-乙氧基新乌头碱、8-乙氧基乌头碱和8-乙氧基次乌头碱;由于反应在加热条件下进行,还有焦乌头碱的产生。Objective： To investigate the pyrolysis mechanism of six alkaloids in Aconitum carmichaeli Debx, and identify their alcoholysates. Methods： HPLC-ESI-MS was used in this study. The first and multi-stage mass spectrum of aconitum alkaloids were obtained in positive ion mode. Results： Through the analysis of multi-stage mass spectrometry, the fragmentation patterns of six alkaloids were illuminated, and the alcoholysates of the diester alkaloids were identified. Conclusion： Three diester alkaloids are alcoholyzed in ethanol and generated three new products respectively, 8 -ethoxy -mesaconitine, 8 -ethoxy -aeonitine and 8 -ethoxy -hypaconitine ; pyraconitine is generated at the same time because the reaction proceeds under heating.

关 键 词：附子 乌头碱 双酯型生物碱 裂解规律 醇解产物 

分 类 号：R285.5[医药卫生—中药学]