Dietary supplementation of Rosmarinus officinalis L.leaves in sheep affects the abundance of rumen methanogens and other microbial populations  

作　　者：Gabriella Cobellis Zhongtang Yu Claudio Forte Gabriele Acuti Massimo Trabalza-Marinucci 

机构地区：[1]Department of Veterinary Medicine,University of Perugia [2]Department of Animal Sciences,The Ohio State University

出　　处：《Journal of Animal Science and Biotechnology》2016年第4期514-521,共8页畜牧与生物技术杂志（英文版）

基　　金：sponsored by the IZSUM 004/09 RC project;funded by the Italian Ministry of Health (Min Sal);supported by a grant from the University of Perugia (Ph D research project in Animal Health, Livestock Production and Food Safety, XXVIII cycle);partially supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2012-67015-19437

摘　　要：Background： Rumen microbiome has a great influence on ruminant health and productivity. Different plant extracts have been tested for their ability to modulate the rumen microbiome to improve feed digestion and fermentation. Among the evaluated plant extracts, essential oils, tannins, and saponins appeared to have positive effects on rumen protein metabolism, volatile fatty acids production, and methane and ammonia production. Methods： The objective of this study was to evaluate the effect of rosemary （Rosmarinus officinalis L.） leaves and essential oils on rumen microbial populations. Four ruminally cannulated sheep were used in a 4x4 Latin square design fed （21 d/period）： 1） a control diet composed of alfalfa hay and concentrate pellet （CTR）, 2） CTR supplemented with 7 g/d/sheep of rosemary essential oil adsorbed on an inert support （EO）, 3） CTR with 10 g/d/sheep of dried and ground rosemary leaves （RL）, and 4） CTR with 10 g/d of dried and ground rosemary leaves pelleted into concentrate （RL pellet）. Abundance of total bacteria, archaea, protozoa, and some select bacterial species or groups was quantified using qPCR, while the community of bacteria and archaea was profiled using denaturing gradient gel electrophoresis. Results： No difference in abundance was noted for total bacteria, protozoa, or Rurninococcus flavefaciens between the control and the treatments, but the rosemary leaves, either in loose form or in pellet, decreased the abundance of archaea and the genus Prevotello （P 〈 0.001）. The rosemary leaves in loose form also decreased （P 〈 0.001） the abundance of Ruminococcus albus and Clostridium aminophilum, while the EO increased （P 〈 0.001） the abundance of Fibrobocter sucdnogenes. The community of bacteria and archaea was not affected by any of the supplements. Conclusions： Being able to affect the abundance of several groups of rumen microbes that are known to be involved in degradation of protein and fiber and production of methane and ammBackground： Rumen microbiome has a great influence on ruminant health and productivity. Different plant extracts have been tested for their ability to modulate the rumen microbiome to improve feed digestion and fermentation. Among the evaluated plant extracts, essential oils, tannins, and saponins appeared to have positive effects on rumen protein metabolism, volatile fatty acids production, and methane and ammonia production. Methods： The objective of this study was to evaluate the effect of rosemary （Rosmarinus officinalis L.） leaves and essential oils on rumen microbial populations. Four ruminally cannulated sheep were used in a 4x4 Latin square design fed （21 d/period）： 1） a control diet composed of alfalfa hay and concentrate pellet （CTR）, 2） CTR supplemented with 7 g/d/sheep of rosemary essential oil adsorbed on an inert support （EO）, 3） CTR with 10 g/d/sheep of dried and ground rosemary leaves （RL）, and 4） CTR with 10 g/d of dried and ground rosemary leaves pelleted into concentrate （RL pellet）. Abundance of total bacteria, archaea, protozoa, and some select bacterial species or groups was quantified using qPCR, while the community of bacteria and archaea was profiled using denaturing gradient gel electrophoresis. Results： No difference in abundance was noted for total bacteria, protozoa, or Rurninococcus flavefaciens between the control and the treatments, but the rosemary leaves, either in loose form or in pellet, decreased the abundance of archaea and the genus Prevotello （P 〈 0.001）. The rosemary leaves in loose form also decreased （P 〈 0.001） the abundance of Ruminococcus albus and Clostridium aminophilum, while the EO increased （P 〈 0.001） the abundance of Fibrobocter sucdnogenes. The community of bacteria and archaea was not affected by any of the supplements. Conclusions： Being able to affect the abundance of several groups of rumen microbes that are known to be involved in degradation of protein and fiber and production of methane and amm

关 键 词：ARCHAEA Essential oil Plant extracts ROSEMARY Rumen microbiome 

分 类 号：S826.5[农业科学—畜牧学]