髓样分化因子88在妊娠期糖尿病发病机制中的作用研究  

Role of Myeloid Differentiation Factor 88 in the Pathogenesis of Gestational Diabetes Mellitus

作　　者：任敏[1] 丛林[1] 袁静[1] 李松[1] 周培[1] 杨琴[1] 高传龙[1] 

机构地区：[1]安徽医科大学第一附属医院,安徽合肥230022

出　　处：《实用妇产科杂志》2016年第11期854-858,共5页Journal of Practical Obstetrics and Gynecology

基　　金：安徽省自然科学基金(编号:1208085MH1720)

摘　　要：目的:通过调控孕妇单核细胞中髓样分化因子88(My D88)的表达水平,研究My D88在妊娠期糖尿病(GDM)发病机制中的作用。方法:30例正常妊娠孕妇为正常组,30例GDM患者为GDM组,两组每个样本处理均相同,每个样本各分为4个组,分别为未处理组、LPS组、ST2825组、LPS+ST2825组。采用Western blot法检测并比较各组单核细胞中My D88及核转录因子-κB(NF-κB)/p65的表达量,分析各组中My D88与NF-κB/p65的相关性;采用ELISA法检测并比较各组培养液中肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1)、白介素-10(IL-10)水平。结果:①正常组和GDM组组内的比较:LPS组、ST2825组的My D88及NF-κB/p65的表达水平与同组未处理组相比,差异有统计学意义(P<0.05);LPS+ST2825组的My D88及NF-κB/p65的表达水平明显低于同组的LPS组(P<0.05)。正常组与GDM组组间比较:GDM组各处理组My D88及NF-κB/p65的表达水平均较正常组相同处理组明显升高(P<0.05)。②两组中除未处理组外,其他处理组的My D88与NF-κB/p65的表达水平均呈正相关关系(P<0.05)。③GDM组中未处理组和LPS组中的细胞因子(TNF-α、IL-1、IL-10)的表达水平均高于正常组中未处理组及LPS组(P<0.05);GDM组中LPS组3个细胞因子的表达水平高于其未处理组(P<0.05),ST2825组3个细胞因子的表达水平低于其未处理组(P<0.05)。GDM组中LPS+ST2825组3个细胞因子的表达水平低于其LPS组(P<0.05)。结论:调控GDM孕妇单核细胞中上游My D88的表达水平,下游NF-κB/p65及细胞因子(TNF-α、IL-1、IL-10)水平发生相应变化,提示My D88可能参与了GDM的发生并在其发病机制中起着重要作用。Objective：To study the pathogenesis of gestational diabetes mellitus by regulating the expression level of myeloid differentiation factor 88 in gravida monocytes. Methods：30 cases of normal pregnant women as normal group,30 patients with GDM for GDM group. Each group was then divided into four subgroups according to the dealing and grouping scheme, namely the untreated group, the LPS group, the ST2825 group and the LPS ＋ ST2825 group. Mononuclear cells via density gradient centrifugation from 15 ml venous blood from every gravida were cultured in vitro by different processing. Western blot was used to detect the expression of MyD88 nuclear factor kappa B（ NF-κB）/p65 in the mononuclear cells and ELISA was used to determine the levels of tumor necrosis factor alpha （TNF-a）, interleukin 1 （ I L-1 ）, interleukin 10 （IL-10） in the supernatant of medium. Resuits ：①The expression level of MyD88, NF-κB/p65 in the LPS and ST2825 group was significantly different compared with the untreated group of normal and GDM group （ P 〈 0.05）. The expression of MyD88, NF-κB/p65 in the LPS ＋ ST2825 group was significantly lower than the LPS group of normal and GDM group（ P 〈 0.05）. The expressions of MyD88,NF-KB/p65 in the each treatment group of GDM group were significantly higher than the normal group（ P 〈0.05）. ②In addition to the untreated group,there was a correlation between the expression of MyD88 and NF-κB/p65 in each treatment group of normal and GDM group（ P 〈 0. 05）. ③The expression levels of cytokines（TNF-α,IL-1 ,IL-10） in the untreated and LPS group of GDM group were higher than the untreated and LPS group of normal group （ P 〈 0.05）. The three cytokines expression in LPS group of GDM group was higher than the untreated group（ P 〈 0. 05）and the ST2825 group was below the untreated group （P 〈 0. 05）. The expression of three cytokines in the LPS ＋ ST2825 group was lower than the LPS group in GDM group（ P 〈 0.05 ）. Conclusions： The e

关 键 词：妊娠期糖尿病 髓样分化因子88 细胞因子 脂多糖刺激 ST2825抑制 

分 类 号：R714.256[医药卫生—妇产科学]