不同等级厚朴饮片质量评价指标的系统聚类分析  

Hierarchical Cluster Analysis of Quality Evaluation Index of Magnoliae Officinalis Cortex with Different Grades

作　　者：翁德会 刘先琼[2] 许腊英[2] 沈雨[2] 胡慧芬[2] 

机构地区：[1]武汉华夏理工学院生物与制药学院,武汉430223 [2]湖北中医药大学药学院,武汉430065

出　　处：《中国实验方剂学杂志》2016年第23期6-10,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局中医药行业科研专项(201007012-2);武汉华夏理工学院校级科研基金项目(16001)

摘　　要：目的：建立厚朴饮片质量评价指标的聚类分析,规范该饮片的质量规格。方法：测定不同产地及同一产地不同树龄厚朴饮片的水溶性浸出物、醇溶性浸出物、挥发油、厚朴酚与和厚朴酚的含量,并对其进行不同类间距离聚类分析。结果：厚朴饮片的水溶性浸出物、醇溶性浸出物、挥发油及总酚的含量与厚朴的级别呈正相关。厚朴饮片可分为三个等级,一级饮片：规格为厚度4-6 mm,挥发油质量分数〉0.7%,水溶性浸出物质量分数〉10%,醇溶性浸出物质量分数〉20%,厚朴酚与和厚朴酚总质量分数〉15%。二级饮片：规格为厚度2-4 mm,挥发油质量分数〉0.35%,水溶性浸出物质量分数〉5%,醇溶性浸出物质量分数〉8%,厚朴酚与和厚朴酚总质量分数〉7%。三级饮片：低于以上各指标值的均归为此类。结论：利用质量指标评价厚朴饮片与传统分级存在一定的相关性,为综合评价厚朴饮片质量规格提供了新思路。Objective： To set up the hierarchical cluster analysis of quality evaluation index of Magnoliae Officinalis Cortex with different grades, and standardize its quality standard. Method： Contents of water soluble extract,alcohol soluble rxtract,volatile oil,magnolol and honokiol in Magnoliae Officinalis Cortex from different origin and different ages with the same origin were determined, then them were investigated by cluster analysis. Result： Contents of water soluble extract,alcohol soluble extract,volatile oil and total phenol were positively correlated with the level of Magnoliae Officinalis Cortex. By the analysis of hierarchical cluster,the three hierarchies were be proposed, such as premium grade with thickness of 4-6 mm, volatile oil content〉 0. 7%,water soluble extract 〉10%,alcohol soluble extract 〉 20%,total content of magnolol and honokiol 15%; the second grade with thickness of 2-4 mm,volatile oil content 〉 0. 35%,water soluble extract 〉5%,alcohol soluble extract 8%,total content of magnolol and honokiol 〉 7%; the third grade with every index was less than the above. Conclusion： There is a certain correlation with traditional grading and quality index evaluation for Magnoliae Officinalis Cortex.

关 键 词：饮片等级 厚朴 浸出物 挥发油 有效成分 聚类分析 厚朴酚 

分 类 号：R282.4[医药卫生—中药学] R284.1[医药卫生—中医学]