主成分和聚类分析法对不同产地半枝莲的综合质量评价  

Comprehensive Quality Evaluation of Scutellaria barbata Different Habitats by Pricipal Component Analysis and Cluster Analysis Method

作　　者：范菊娣[1] 覃容贵[1] 张珏[1] 李相陵[2] 杨传玉[1] 

机构地区：[1]贵州医科大学药学院,贵阳550004 [2]贵州医科大学医药卫生管理学院,贵阳550004

出　　处：《中国实验方剂学杂志》2016年第23期40-44,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科技厅中药开发专项(黔科合【2012】5001-4号);贵州省科技厅社会发展攻关项目(黔科合LG字【2012】029号)

摘　　要：目的：综合比较并评价不同产地半枝莲的质量。方法：采用RP-HPLC法,紫外分光光度法,测定不同产地半枝莲中野黄芩苷、大波斯菊苷、总黄酮和多糖的含量;并以这4个成分为指标,运用主成分分析和聚类分析对13个产地的半枝莲进行综合质量评价。结果：不同产地半枝莲中野黄芩苷、大波斯菊苷、总黄酮和多糖含量有明显差异,其中总黄酮和多糖含量丰富,分别在18.56-74.67,19.67-47.3 mg·g-1,所有样品均达到药用要求。主成分分析发现,选择2个因子（F1,F2）即可对半枝莲药材进行综合评价,其综合评价函数为F=0.581 28F1＋0.278 82F2,其综合评价得分在-0.66-1.68。以贵州锦屏、云南、贵州镇远半枝莲药材样品的质量综合评价较好。结论：不同产地及生长环境对半枝莲质量有影响,该研究可为半枝莲质量评价及种质资源筛选提供一定的参考依据。Objective： Compare and evaluate the quality of Scutellaria barbata from different habitats.Method： The content of scutellarin,cosmosiin,total flavonoids and polysaccharides were determined by RP-HPLC and ultra-violet spectrolhotometry. Through the content of 4 indicated ingredients as the index,the quality of S. barbata was assessed by principal componentanalysis and cluster analysis method. Result： The difference of the content of scutellarin,cosmosiin,total flavonoids and polysaccharides of S. barbata which from different habitats were quite large. The total flavonoids and polysaccharides were abundance in S. barbata,which were 18. 56-74. 67mg·g-1 and 19. 67-47. 3 mg·g-1 respectively. All the samples are up to the medical requirements. PCA concluded that two factors（ F1,F2） could be used to evaluate the quality of S. barbata. The function is following F = 0. 581 28F1＋ 0. 278 82F2,the scores of different habitats of S. barbata were from-0. 66-1. 68. The quality of S. barbata in the Jinping of Guizhou,Yunnan and Zhenyuan of Guizhou is the best respectively. Conclusion：The habitats and growing environment have some effect on the quality of S. barbata,this research can provide some reference for the germplasm resources and quality evaluation of S. barbata.

关 键 词：半枝莲 主成分分析 聚类分析 质量评价 

分 类 号：R282[医药卫生—中药学]