产酸模素苷及白藜芦醇苷的毛脉酸模内生真菌筛选  

Screening for Endophytic Fungi of Rumex gmelini Producing Nepodin-8-O-β-D-Glucopyranoside and Polydatin

作　　者：丁常宏[1] 郭美[1] 王谦博[2] 王振月[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]广东药学院附属第一医院药学部,广州510080

出　　处：《中国实验方剂学杂志》2016年第23期45-48,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30970300);黑龙江省自然科学基金面上项目(C2016053);黑龙江中医药大学科研基金项目(015128)

摘　　要：目的:在前期的研究工作中已从不同产地分离得到毛脉酸模内生真菌278株,以这些内生真菌为研究对象,寻找能够通过自身发酵产生酸模素苷、白藜芦醇苷的内生真菌。方法:通过HPLC检测内生真菌的次生代谢产物,以酸模素苷、白藜芦醇苷为参照,筛选能够产生酸模素苷、白藜芦醇苷的内生真菌,并测定其含量。结果:发现其中的11株内生真菌的次生代谢产物中含有酸模素苷,5株次生代谢产物中含有白藜芦醇苷,其中内生真菌tq-s1的次生代谢产物中酸模素苷的质量分数最高(530μg·g-1),且白藜芦醇苷的质量分数也最高(224μg·g-1)。结论:内生真菌的种类和其次生代谢产物的形成受到地理位置的影响,对于酸模素苷和白藜芦醇苷的高产菌种tq-s1,可以进行开发利用的研究。Objective： In the previous study,278 strains of endophytic fungi were separated from Rumex gmelini in different origins. These endophytic fungi were taken as the research object to screen out endophytic fungi that could produce nepodin-8-O-β-D-glucopyranoside and polydatin through fermentation.Method： HPLC was used to detect secondary metabolites of endophytic fungi,nepodin-8-O-β-D-glucopyranoside and polydatin were used as reference to screen out endophytic fungi that could produce nepodin-8-O-β-Dglucopyranoside and polydatin,and determine their content. Result： Eleven strains of endophytic fungi whose secondary metabolites containing nepodin-8-O-β-D-glucopyranoside were found,meanwhile 5 strains could produce polydatin. Among them,fungi tq-s1 had the highest mass fraction of nepodin-8-O-β-D-glucopyranoside（ 530μg·g-1） and polydatin（ 224 μg·g-1） in its secondary metabolites. Conclusion： Endogenous fungal species and formation of their secondary metabolites were affected by geographical location. Further studies shall be conducted on the development and utilization of high-yield strain tq-s1 of both nepodin-8-O-β-D-glucopyranoside and polydatin.

关 键 词：毛脉酸模 内生真菌 酸模素苷 白藜芦醇苷 

分 类 号：R282[医药卫生—中药学]