霉茶蛋白对自发性高血压大鼠(SHR)血压和血管的作用  

Effect of Meicha Protein on Blood Pressure and Blood Vessels of Spontaneously Hypertensive Rats

作　　者：郑国华[1] 刘洋洋[1] 吴勇[1] 谭永霞[1] 胡俊杰[1] 田先翔[1] 张宝徽[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室老年病中药新产品湖北省协同创新中心,武汉430065

出　　处：《中国实验方剂学杂志》2016年第23期116-120,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173502)

摘　　要：目的:研究霉茶蛋白连续给药后对自发性高血压大鼠(SHR)血压和血管的作用及机制。方法:将40只雄性SHR随机分为模型组(等容量蒸馏水),复方罗布麻片阳性药组(50 mg·kg^(-1)),霉茶蛋白低、高剂量(70,140 mg·kg^(-1))组。ig容量均为10 m L·kg^(-1)体重,各组每天上午和下午各ig给药1次,连续给药7周。测定给药前大鼠尾动脉血压,并在给药后每周测1次大鼠尾动脉血压。末次给药后取血并取其胸主动脉,检测血清一氧化氮(NO)和内皮素^(-1)(ET^(-1))的含量,实时荧光聚合酸链式反应(Real-time PCR)测定血管内皮型一氧化氮合酶(e NOS),血管紧张素转换酶(ACE),血管紧张素Ⅱ(AngⅡ),原癌基因(c-Myc),p27,B细胞淋巴瘤/白血病-2(Bcl-2)mRNA的表达。结果:与模型组比较,霉茶蛋白低、高剂量组从第2周开始可显著降低SHR的血压值,能够升高血清中NO的含量,降低ET^(-1)的含量,抑制基因c-Myc,Bcl-2,ACE mRNA的表达,促进e NOS mRNA的表达(P<0.05,P<0.01),但对p27 mRNA无影响。结论:霉茶蛋白对SHR有显著降压作用,对血管有保护作用,其机制可能与改变其血清中NO和ET^(-1)水平,以及与血管基因c-Myc,Bcl-2,ACE,AngⅡ和e NOS的表达有关。Objective： To study the effect of Meicha protein on blood pressure and blood vessels of spontaneously hypertensive rats（ SHR） rats after continuous administration,and explore its mechanism. Method：Totally 40 male SHR were randomly divided into model（ equal volume of distilled water）,positive control group（ compound Kendir leaves tablets,50 mg·kg-1）,and low and high-dose Meicha protein groups（ 70,140 mg·kg-1）. Each group was intragastrically administrated with drugs（ 10 m L·kg-1） once in the morning and afternoon for 7 weeks. The blood pressure at rat tail artery was measured before administration,and after administration once a week. The blood and thoracic aorta were collected after the last administration to detect the content of nitric oxide（ NO） and endothelin-1（ ET-1） in the serum,and the mRNA expressions of endothelial nitric oxide synthase（ e NOS）,angiotensin converting enzyme（ ACE）,angiotensin Ⅱ（ Ang Ⅱ）,c-Myc,p27 and B cell lymphoma/leukemia-2（ Bcl-2） gene in the blood vessels. Result： Compared with model group,low and high-dose Meicha protein groups can significantly decrease the blood pressure of SHR since the second week,increase the content of NO,reduce the content of ET-1,inhibite the expressions of gene c-Myc,Bcl-2 and ACE,and enhance the mRNA expression of e NOS（ P〈 0. 05,P〈 0. 01）,but with no effect on p27 mRNA. Conclusion： Meicha protein has significant antihypertensive and vascular-protective effects on SHR. The mechanism may be correlated with the changes in the level of NO and ET-1 in the serum,and the expressions of gene c-Myc,Bcl-2,ACE,Ang Ⅱ and e NOS in the blood vessel.

关 键 词：霉茶蛋白 自发性高血压大鼠 胸主动脉 血管重塑 降压机制 

分 类 号：R285.5[医药卫生—中药学]