萱草花总黄酮对小鼠急性酒精性肝损伤保护作用及机制探讨  

Protection Mechanism of Total Flavones from Hemerocallis Fulva on Alcohol-induced Liver Injury in Mice

作　　者：徐博[1] 吴畏难[2] 李传甲[2] 陈雪[1] 赵东海[1] 李长花 沈楠[1] 

机构地区：[1]吉林医药学院基础医学院,长春132013 [2]吉林市中心医院,长春132011 [3]吉林省靖宇县生殖保健医院,吉林靖宇135200

出　　处：《中国实验方剂学杂志》2016年第23期139-143,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：吉林省教育厅基金项目(2013359)

摘　　要：目的:观察萱草花总黄酮对小鼠急性酒精性肝损伤的保护作用,并探讨其可能的作用机制。方法:60只小鼠随机分为正常组、模型组、联苯双酯组和萱草花总黄酮低、中、高剂量组。采用一次性灌胃乙醇的方法建立急性酒精性肝损伤模型。观察萱草花总黄酮对各组血清丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)活性以及肝组织超氧化物歧化酶(SOD),丙二醛(MDA),谷胱甘肽过氧化物酶(GPX)以及还原型谷胱甘肽(GSH)水平的影响,并观察肝组织病理改变;蛋白质免疫印迹法(Western blot)检测肝细胞B淋巴细胞瘤-2(Bcl-2),Bcl-2相关X蛋白(Bax)蛋白的表达。结果:与模型组比较,萱草花总黄酮可明显降低血清ALT,AST活性(P<0.05);降低肝组织中MDA含量,提高SOD,GPX,GSH活性(P<0.05,P<0.01)。病理学检查可见,与模型组比较,萱草花总黄酮中、高剂量组可明显减轻肝细胞变性和坏死的程度,改善肝组织的病理学改变。Western blot结果可见萱草花总黄酮组Bcl-2蛋白表达增加(P<0.05,P<0.01);Bax蛋白表达减少,Bax/Bcl-2降低(P<0.05,P<0.01)。结论:萱草花总黄酮对乙醇所致小鼠急性酒精性肝损伤具有明显的保护作用,其作用机制可能与其抗氧化作用及调节凋亡相关蛋白Bcl-2,Bax的表达有一定关联。Objective： To study the protection effect of total flavones from the hemerocallis fulva（ TFHF） on alcohol-induced liver injury in mice and explore their possible pharmacological mechanisms. Method： A total of 60 mice were randomly divided into normal group,model group,bifendate group and TFHF low dose,middle dose,and high dose groups. Acute hepatic injury models were established by once gavage administration of alcohol. Levels of alanine aminotransferase（ ALT） and aspartate aminotransferase（ AST） in serum,as well as superoxide dismutase（ SOD）,malondialdehyde（ MDA）,glutathione peroxidase（ GPX） and glutathione（ GSH） levels in liver tissues were assayed and compared. The pathological changes in liver tissues were observed,and the expression levels of Bcl-2and Bax were detected by Western blot. Result： As compared with the model group,TFHF could significantly reduce serum ALT and AST levels（ P〈 0. 05）,decrease the MDA content in liver tissues and increase the activities of SOD,GSH and GPX（ P 〈 0. 05,P 〈 0. 01）. Pathological examination showed that as compared with the model group, TFHF middle dose group and high dose group could significantly alleviate the liver cell degeneration and necrosis,and improve the pathological changes of hepatic tissues. Western blot results showed that the protein expression level of Bcl-2 was significantly increased in TFHF groups（ P 〈 0. 05,P 〈 0. 01）,whereas the protein expression of Bax was decreased and Bax/Bcl-2 was also decreased（ P 〈 0. 05,P 〈 0. 01）.Conclusion： TFHF may have a significant protection effect on alcohol-induced liver injury in mice,and the mechanism may be associated with its antioxidation effect and regulating the expression levels of Bcl-2 and Bax.

关 键 词：萱草花总黄酮 酒精性肝损伤 抗氧化作用 B淋巴细胞瘤-2 Bcl-2相关X蛋白 

分 类 号：R285.5[医药卫生—中药学]