道地通管汤对输卵管炎性大鼠信号转导通路相关蛋白作用机制的研究  

Research on Protein Mechanism Related to Signal Transduction Pathway of “Dao Di Tong Guan Tang” in Rats of Salpingitic

作　　者：符丽[1] 潘小敏[1] 吴媛媛[2] 罗志娟[2] 刘琴[1] 韦欣宏[1] 李芳艳[1] 

机构地区：[1]广西中医药大学,广西南宁530001 [2]广西中医药大学附属瑞康医院,广西南宁530001

出　　处：《中华中医药学刊》2016年第12期2890-2893,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81360594)

摘　　要：目的:通过实验观察道地通管汤对输卵管炎性大鼠信号转导通路TGF-β1、Smad2、Smad3、Smad7蛋白的影响,探讨其作用机制。方法:80只SD雌性大鼠随机抽取12只做正常组,剩余大鼠采用混合菌制成输卵管炎性模型后将其分为模型组、康妇消炎栓组及道地通管汤低、中、高剂量组。每组采用相应的干预措施灌肠,正常组和模型组予生理盐水0.2 m L/100 g·d,阳性对照组予康妇消炎栓水溶剂0.2 m L/100 g·d,道地通管汤低、中、高剂量组分别予0.35 g/100 mg·d、0.7 g/100 mg·d、1.4 g/100 g·d。于灌肠第15天、30天采集输卵管,蛋白印迹法(Western blotting)检测输卵管组织TGF-β1、Smad2、Smad3及smd7蛋白的表达。结果:1与正常组比较,模型组及各治疗组TGF-β1、Smad2、Smad3的表达量增加,Smad7的表达量减少(P<0.05);2与模型组比较,各治疗组能不同程度降低模型大鼠输卵管组织中TGF-β1、Smad2、Smad3表达量,增加Smad7的表达量,以道地通管汤高剂量最为突出(P<0.05);3各组自身比较,模型组30 d时TGF-β1、Smad2、Smad3的表达量高于15 d的,而Smad7的表达量低于15 d(P<0.05);各治疗组给药30 d时TGF-β1、Smad2、Smad3的表达量低于给药15 d的,而Smad7的表达量高于给药15 d,以道地通管汤高剂量最为突出(P<0.05)。结论:道地通管汤对输卵管炎性大鼠有明显的治疗作用,其机制可能通过TGF-β1/Smads信号通路下调输卵管组织中TGF-β1、Smad2、Smad3表达,上调Smad7的表达,抑制炎症反应、减轻输卵管组织纤维化,从而改善输卵管组织结构。Objective： To observe the effects of ＂ Dao Di Tong Guan Tang＂ on signal transduction pathway—protein TGF-β1,Smad2,Smad3 and Smad7 in salpingitis rat,and to explore its mechanism.Methods： 12 out of 80 SD female rats were randomly selected into normal group,and the remaining rats were divided into model group,Kang Fu anti-inflammatory group,low dose group,middle dose group and high dose group of ＂ Dao Di Tong Guan Tang＂.Each group was applied with enema by corresponding interventions.Normal group and model group were given saline by 0.2 m L/100g·d,positive control group was treated with Kang Fu anti-inflammatory suppository water solvent by 0.2 m L/100 g·d,and the administration dosages of the low,middle,and high dose groups of ＂ Dao Di Tong Guan Tang＂ were respectively 0.35 g/100 mg·d,0.7 g/100 mg·d and 1.4 g/100 g·d.Western blotting was used to detect the expression of protein TGF-β1,Smad2,Smad3 and smd7 in fallopian tubes on the 15 th and 30 th day of enema.Results： 1Compared with normal group,an increasing expression of TGF-β1,Smad2,Smad3 and a decreasing expression of Smad7 were observed in model group and treatment groups（P〈0.05）; 2Compared with model group,a decreasing expression of TGF-β1,Smad2,Smad3 and an increasing expression of Smad7 in fallopian tubes of model rats were observed in all treatment groups,and that of in the high dose group of ＂Dao Di Tong Guan Tang＂is the most prominent（P〈0.05）.3In self-comparison of each group on the 30 th day,a higher expression of TGF-β1,Smad2 and Smad3 and a lower expression of Smad7 were observed on the 15 th day in model group.（P〈0.05）.A lower expression of TGF-β1,Smad2 and Smad3 and a higher expression of Smad7 were observed in all treatment groups on the 15 th day after administration,and that of in the high dose group of ＂ Dao Di Tong Guan Tang＂ is the most prominent（P〈0.05）.Conclusion： ＂ Dao Di Tong Guan Tang＂ has significant therapeutic effect on the salpingitis rats,and the mechanism is proba

关 键 词：输卵管炎性疾病 道地通管汤 TGF-β1/Smads信号通路 TGF-β1 SMAD2 SMAD3 SMAD7 

分 类 号：R285.5[医药卫生—中药学]