百合知母汤对CUMS抑郁症大鼠行为及海马中BDNF/TrKB表达变化的影响  

Effect of Baihe Zhimu Decoction to Depression on Behavior and Expression of BDNF/TrKB in Hippocampus

作　　者：袁丽[1] 李德顺[2] 吴建红[2] 韩永明[2] 

机构地区：[1]湖北中医药大学,湖北武汉430065 [2]湖北中医药大学基础医学院,湖北武汉430065

出　　处：《中华中医药学刊》2016年第12期2941-2944,I0035,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：湖北省教育厅科研计划重点项目(D20142001)

摘　　要：目的:研究百合知母汤对抑郁症大鼠行为学及海马组织中脑神经营养因子(BDNF)、络氨酸激酶受体B(Tr KB)表达变化的影响。方法:50只大鼠按照体重随机分为正常对照组、模型组、氟西汀组、百合知母汤中剂量组、百合知母汤高剂量组,每组10只;除正常对照组外,其余4组均采用采用慢性轻度不可预见性温和刺激(CUMS)结合孤养的方法制造抑郁症模型,共计21 d;造模结束后,氟西汀组给予氟西汀混悬液(1.8 mg·kg-1)、百合知母汤中、高剂量组给予百合知母汤(1.5 g·kg-1、3.0 g·kg-1),正常对照组、模型组均给予等体积生理盐水,每日1次,共计28 d;分别于实验前1天、第21天、49天检测各组大鼠糖水偏好度、5 min内悬尾不动的时间;行为学检测完毕后,处死动物,检测肾上腺指数,海马组织HE染色,高倍显微镜下观测各组病理切片神经元细胞形态变化;q-PCR法检测各组大鼠海马组织BDNF、Tr KBm RNA表达的变化。结果:百合知母汤高、中剂量组、氟西汀组大鼠糖水偏好度提高、5 min内悬尾不动的时间降低,肾上腺指数降低,与模型组比较,差异具有统计学意义(P<0.05或P<0.01);各给药组海马组织切片中,神经元细胞数量增多、排列结构和层次明显改善;百合知母汤高剂量组中大鼠海马组织中BDNF、Tr KB m RNA的表达上调(与模型组比较,P<0.05)。结论:百合知母汤能改善抑郁症大鼠的抑郁状态,具有增强海马组织神经元再生和修复的功能,其机制与抑制HPA轴亢进,促进BDNF、Tr KB m RNA的表达有关。Objective： To explore the effect of Baihe Zhimu Decoction（BZD） on behavior of depression rats whether related to the expressions of BDNF and Tr KB in hippocampus.Methods： Fifty rats were divided into five groups randomly named as control group,model group,Fluoxetine group,high and moderate dosage of BZD group.Firstly,the depression model was made by using unpredictable chronic mild stimulation combined with isolated keeping for 21 days.All rats were given either saline or Fluoxetine and BZD respectively for 28 days.Secondly,the sugar preference rate（SPR） and tail-suspension time within 5 min（TST） were observed before and on 21^ stand 49^thday.Adrenal index（AI）,histology and the expressions of BDNF and Tr KB（by using q-PCR） in hippocampus tissue were observed.Results： In groups of BZD,the SPR levels were increased.TST and AI were decreased significantly contrast to those of model group（P〈0.05,P〈0.01）.The pathological damage was repaired of a great degree and the expressions of BDNF and Tr KB in hippocampus were both increased after 28 days＇ treatment.Conclusion： BZD had significant effect on depression rats via depressing the hyperthyroidism of HPA axis,which increases the expression of BDNF and Tr KB in hippocampus.

关 键 词：抑郁症 脑源性神经营养因子 络氨酸激酶受体B 百合知母汤 

分 类 号：R285.5[医药卫生—中药学]