人参皂苷Re对慢性酒精中毒大鼠学习、记忆能力的影响及机制  

Effects and Mechanism of Ginsenoside Re on Learning and Memory Abilities of Rats with Chronic Alcoholism

作　　者：陈荻 李阳[1] 田梦[1] 王颖娴[1] 郝蕴超 刘晓丽 刘耀武[3,2] 

机构地区：[1]徐州医学院临床医学系,江苏徐州221004 [2]江苏省新药研究与临床药学重点实验室,江苏徐州221004 [3]徐州医学院药理学教研室,江苏徐州221004

出　　处：《中华中医药学刊》2016年第12期2974-2977,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省高校“青蓝工程”项目(2014);江苏省高校优势学科建设工程项目;徐州医学院“振兴计划”项目(2012)

摘　　要：目的:探讨人参皂苷Re对慢性酒精中毒大鼠学习、记忆能力的影响及其可能机制。方法:雄性SD大鼠除正常组外,慢性酒精中毒组、人参皂苷Re低、高剂量治疗组(50,100 mg/kg,ig.)进行模型诱导,每组10只。8周后采用Morris水迷宫评价大鼠的学习、记忆功能,紫外分光光度法检测前额皮质部位乙酰胆碱酯酶(ACh E)和单胺氧化酶(MAO)活性,ELISA法检测IL-1β,TNF-α含量。结果:与模型组相比,人参皂苷Re低、高剂量组大鼠的逃避潜伏期均显著缩短(P<0.05),穿越平台次数均显著增加(P<0.05),高剂量组大鼠在原平台所在象限游泳时间的百分比表现出明显增加的趋势。同时,与模型组相比,人参皂苷Re高剂量组大鼠大脑皮质ACh E活性、MAO活性均明显降低(P<0.01),IL-1β含量明显降低(P<0.05),TNF-α含量表现出明显降低的趋势;低剂量组大鼠的MAO活性和IL-1β含量明显降低(P<0.05)。结论:人参皂苷Re对慢性酒精中毒引起的大鼠学习、记忆能力损害有明显改善作用,这可能与其抑制脑内ACh E和MAO活性及抗炎作用有关。Objective： To investigate effects of ginsenoside Re（Re） on learning and memory abilities of rats with chronic alcoholism and the potential mechanisms.Methods： Male rats were randomly divided into normal（C）,model（M）,Re low dose（Re-L,50 mg/kg）,Re high dose（Re-H,100 mg/kg） groups,and 10 rats in each group.Except normal group,other groups were subjected to intragastric administration 8 weeks with progressively increased concentration of ethanol to create the model of chronic alcoholism.Learning and memory abilities were evaluated with Morris water maze.Prefrontal cortices were collected for the determination of acetylcholinesterase（ACh E） and monoamine oxidase（MAO）activities with ultraviolet spectrophotometry,interleukin-1β（IL-1β） and tumor necrosis factor（TNF-α） levels by enzyme linked immunosorbent assay（ELISA）.Results： Both low and high doses of Re significantly improved cognitive performances of chronic alcoholism rats,evidenced by a decrease in escape latency and an increase in numbers of crossing the platform（each P〈0.05）,and high dose of Re showed an increased tendency for the percentage of time spent in the target quadrant.High dose of Re induced significant decreases in ACh E and MAO activities as well as IL-1β level in prefrontal cortex（P〈0.05 or P〈0.01）,and a decreased tendency for TNF-α level,while low dose of Re remarkably reduced MAO activity and IL-1β level（both P〈0.01）.Conclusion： Ginsenoside Re can markedly ameliorate the cognitive decline in rats with chronic alcoholism,likely at least partly due to its inhibitory effects on ACh E and MAO activities as well as inflammation.

关 键 词：人参皂苷RE 慢性酒精中毒 认知功能 乙酰胆碱酯酶 单胺氧化酶 炎症 

分 类 号：R285.5[医药卫生—中药学]