大黄牡丹汤改善DSS诱导的溃疡性结肠炎小鼠炎症状态及其贫血症  

Rhubarb Peony Decoction Ameliorate DSS-induced Ulcerative colitis and It's Associated Anemia in Mice

作　　者：温如燕[1,2] 罗霞[1] 郑彦懿 周联[1] 廖海锋 刘琦[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广西中医药大学,广西南宁530200

出　　处：《中华中医药学刊》2016年第12期2998-3001,I0040,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(2015A030313344)

摘　　要：目的:研究大黄牡丹汤对葡聚糖硫酸钠诱导的溃疡性结肠炎(ulcerative colitis,UC)小鼠的治疗作用及其相关的贫血症的改善作用。方法:设正常组、模型组、美沙拉嗪组、大黄牡丹汤低剂量组、大黄牡丹汤中剂量组和大黄牡丹汤高剂量组,通过疾病活动指数(DAI)、结肠病理损伤评分、结肠髓过氧化物酶(myeloperoxidase,MPO)活性、外周血中性粒细胞数量、红细胞总数及血红蛋白含量观察大黄牡丹汤对UC小鼠的治疗效果及对炎症性肠病相关的贫血症的影响。结果:模型组小鼠DAI评分显著升高,结肠横切面显著增厚,大量炎症性细胞浸润,溃疡部位明显,隐窝和肠腺减少,病理组织学评分显著升高,外周血中性粒细胞显著增多,红细胞数减少,血红蛋白含量显著减少。美沙拉秦可降低UC小鼠DAI评分、组织病理学评分,但对UC小鼠结肠增厚、中性粒细胞增多及红细胞及血红蛋白减少作用不明显。大黄牡丹汤个剂量组均能降低DAI评分、组织病理学评分、结肠MPO活性。大黄牡丹汤中剂量组和高剂量组还可显著防止结肠组织增厚,维持其结构的完整,显著抑制外周血中性粒细胞的增多和提高红细胞数及血红蛋白含量。结论:大黄牡丹汤可以改善葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠炎症状态及其贫血症。Objective： To study the therapeutic effect of Rhubarb Peony Decoction（RPD） on dextran sulfate sodium（DSS）-induced ulcerative colitis（UC） and UC-associated anemia in mice.Methods： Set the control group,ulcerative colitis group,mesalazine group,low dose group of Rhubarb Peony Decoction（RPDL）,,middle dose group of Rhubarb Peony Decoction（RPDH） and high dose group of Dahuang Mudan Decoction（DMDH）.The treatment effects were studied by disease activity index（DAI）,the length of the colon,histological analysis,colon pathological lesion scores,colon myeloperoxidase（MPO） activity as well as the number of peripheral blood neutrophils,the total number of red blood cells and hemoglobin content.Results： Compared with the normal group,DAI scores of ulcerative colitis group were significantly increased.The cross-section of the colon was significantly thicker and accompanied with a large number of inflammatory cell infiltration,multifocal ulceration,crypt and intestinal glands reduced,histological scores and colon MPO activity were significantly higher;neutrophil cells in peripheral blood increased significantly;the number of red blood cells and hemoglobin all significantly reduced.Compared with the DSS group,mesalazine reduced DAI scores and histological scores,but the effects on colon thicken,neutrophils increase,red blood cells and hemoglobin reduce in UC mice were not obvious.RPD all doses groups can lower DAI scores,histological scores and colon MPO activity.RPDM and RPDH also can significantly prevent colon tissue thickening,maintain the integrity of its structure,inhibit neutrophils increase in peripheral blood,increase red blood cells and hemoglobin content significantly.Conclusion： Rhubarb Peony Decoction has a therapeutic effect on DSS-induced ulcerative colitis and UC-associated anemia in mice.

关 键 词：大黄牡丹汤 溃疡性结肠炎 葡聚糖硫酸钠 贫血症 治疗作用 小鼠 

分 类 号：R285.5[医药卫生—中药学]