趋化因子及黏附因子在重症实验性自身免疫性神经炎模型中作用的研究  

Roles of chemokines and adhesion molecules of severe experimental autoimmune neuritis

作　　者：李慧[1] 吴云[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经内科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2016年第5期407-410,共4页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金资助项目(D200655)

摘　　要：目的研究趋化因子、黏附因子与重症实验性自身免疫性神经炎(experimental autoimmune neuritis,EAN)大鼠模型发病的关系。方法采用400μg P257-81多肽和弗氏完全佐剂(FCA)的混合乳液诱导重症EAN大鼠模型,每天对其进行临床评估,在致敏后第20 d及第45 d分别处死大鼠,并取其脾脏和双侧坐骨神经。RT-PCR方法检测坐骨神经中趋化因子单核趋化蛋白1(monocyte chemotactic protein 1,MCP-1/CCL2)及细胞间黏附分子(intercellular adhesion molecule,ICAM-1)的表达,同时进行坐骨神经病理学检查。结果在致敏后第20 d,EAN大鼠瘫痪症状高峰期,坐骨神经病理学显示:大量炎性细胞浸润;坐骨神经RT-PCR检测显示:CCL2表达较正常对照组明显增高。在致敏后45天,EAN大鼠瘫痪症状仍迁延不愈,病理学显示:坐骨神经中仍有炎性细胞浸润;CCL2维持在较高表达水平。ICAM-1在对照组、致敏后20 d组及45 d组中表达无明显差异。结论 CCL2可能与重症EAN大鼠症状迁延不愈及炎性细胞持续浸润有关。Objective To study the role of chemokines and adhesion molecules of severe experimental autoimmune neuritis （EAN）. Methods Severe experimental autoimmune neuritis was induced by immunization with P257-81 peptide and Freund＇ s complete adjuvant （FCA）. 20 d and 45 d after immunization, the rats were sacrificed for fresh spleen, sciatic nerves. The expression levels of monocyte chemotaetic protein 1 （ CCL2 ） and intercellular adhesion molecule （ICAM-1） were examined by RT-PCR, the condition of inflammatory cell infiltration and demyelination of peripheral nerves was observed by HE staining at the same time. Results The Lewis rats got the peak clinical weakness 20 d after immunization, HE staining results showed a large number of immune cells infiltration into sciatic nerves, the expression of CCL2 was higher than the normal control groups. The rats of severe EAN maintained the relative high scores, only CCL2 keeped high expression 45 d after immunization. In addition, ICAM-I showed no difference among the three groups after immunization and normal Lewis rats. Conclusion CCL2 may play an important role in the process of severe EAN, it participates the course of inflammatory cell infiltrating.

关 键 词：吉兰巴雷综合征 实验性自身免疫性神经炎 趋化因子单核趋化蛋白1 细胞间黏附分子 

分 类 号：R593.2[医药卫生—内科学]